In vitro inhibitory activities of sugarcane extract on avian Eimeria sporozoites

Abstract

The current <i>in vitro</i> study aimed to investigate the effects of a processed sugarcane extract on the viability of avian <i>Eimeria</i> sporozoites. Treatments were applied to hatched sporozoites: 1) without additives (no-treatment control); 2) with ethanol; 3) with salinomycin; 4) with Polygain™. All treatments were incubated in RPMI media containing live sporozoites at 37°C for 14 h and then the number of viable sporozoites were counted. Compared to the no-treatment control, Polygain™ decreased (P < 0.001) the counts of <i>E. maxima</i>, <i>E. acervulina</i>, <i>E. bruneti</i>, and <i>E. mitis</i> sporozoites to a level similar to salinomycin (P > 0.05). In conclusion, Polygain™ could be a potential candidate as an anticoccidial agent.