Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/30909
Title: Detection and Quantification of Clostridium perfringens and Eimeria spp. in Poultry Dust Using Real-Time PCR Under Experimental and Field Conditions
Contributor(s): Ahaduzzaman, Md  (author); Keerqin, Chake  (author)orcid ; Kumar, Alip  (author); Musigwa, Sosthene  (author)orcid ; Morgan, Natalie  (author)orcid ; Kheravii, Sarbast K  (author); Sharpe, Sue (author); Williamson, Sarah (author); Wu, Shu-Biao  (author)orcid ; Walkden-Brown, Stephen W  (author)orcid ; Gerber, Priscilla F  (author)orcid 
Publication Date: 2021-03
Early Online Version: 2020-09-09
DOI: 10.1637/aviandiseases-D-20-00084
Handle Link: https://hdl.handle.net/1959.11/30909
Abstract: Infection of poultry with Eimeria spp., the causative agent of coccidiosis, can predispose birds to necrotic enteritis(NE) caused by netB gene–positive strains of Clostridium perfringens. The detection of Eimeria spp., C. perfringens, and netB were examined in settled dust from broiler flocks under experimental and field conditions. Dust samples were collected from settle plates twice weekly from two experimental flocks inoculated with three species of pathogenic Eimeria in 9-day-old chicks, followed by netB gene–positive C. perfringens 5 days later to produce subclinical and clinical NE. A noninoculated flock was sampled weekly from day 0 and served as a control flock. An additional 227 dust samples from commercial broiler flocks were collected at the end-of-batch (6–7 wk of age; one scraped dust sample per flock). In the NE-subclinical and NE-clinical flocks, high levels of Eimeria spp. and C. perfringens were detected after inoculation followed by a gradual decline over time. In the control flock, C. perfringens and netB were detected at low levels. No significant effect of sampling location was evident on Eimeria spp., C. perfringens, and netB load within poultry houses. These results provide evidence that Eimeria spp., C. perfringens, and netB gene copies can be readily measured in poultry dust samples collected in settle plates and may provide an alternative sampling method for monitoring flock coccidiosis and NE status. Further studies are required to assess the utility for such a test in commercial flocks.
Publication Type: Journal Article
Source of Publication: Avian Diseases, 65(1), p. 77-85
Publisher: American Association of Avian Pathologists, Inc
Place of Publication: United States of America
ISSN: 1938-4351
0005-2086
Fields of Research (FoR) 2020: 300304 Animal protection (incl. pests and pathogens)
Socio-Economic Objective (SEO) 2020: 100411 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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