Kolakshyapati, Manisha; Bailey, Craig; Sibanda, Terence Zimazile; Morgan, Natalie; Ruhnke, Isabelle

Author(s)

Kolakshyapati, Manisha

Bailey, Craig

Sibanda, Terence Zimazile

Morgan, Natalie

Ruhnke, Isabelle

Publication Date

2019-09

Abstract

The titanium dioxide (TiO2) marker technique is currently widely practiced as a method to evaluate gastrointestinal (GI) passage rate in poultry. However, this method requires sacrificing the animal to obtain digesta samples, is labour-intensive and eliminates the possibility of follow-up studies with the same individual. The aim of this study was to evaluate whether the radiographic methods barium-impregnated polyethylene spheres (BIPS) and barium sulphate (BaSO4) suspension are in agreement with the TiO2 technique and can be used as an alternative method for GI passage rate determination in laying hens. Whole-body radiographs were taken at different time points. Hens of group 3 (n = 55) were orally inoculated with 5 g of feed mixed with 0.15 g TiO2, 5 hens per time point sacrificed, and whole gastrointestinal organs (crop, proventriculus, gizzard, small intestine and large intestine) were collected and analysed for TiO2 content. The average marker passage rate of hens administered BaSO4 was significantly faster than those administered BIPS (gizzard: 15.2 hr vs. 43.2 hr; small intestine: 15.2 hr vs. 38.4 hr, respectively). A greater percentage of BIPS remained in the crop at 0, 0.5, 2, 3 and 8 hr post-inoculation (p.i.) and in the gizzard at 2, 24, 36 and 48 hr p.i. (all p < 0.05) compared to TiO2. In conclusion, the evaluation of the GI transit time is feasible using BIPS, TiO2 and BaSO4. The evaluation of the GI transit time using BIPS and BaSO4 needs further investigation.

Citation

Journal of Animal Physiology and Animal Nutrition, 103(5), p. 1427-1436

ISSN

1439-0396

0931-2439

Link

https://hdl.handle.net/1959.11/30897

Publisher

Wiley-Blackwell Verlag GmbH

Title

Determination of gastrointestinal passage rate using three different markers in laying hens

Type of document

Journal Article

Entity Type

Publication

Author(s)	Kolakshyapati, Manisha Bailey, Craig Sibanda, Terence Zimazile Morgan, Natalie Ruhnke, Isabelle
Publication Date	2019-09
Abstract	The titanium dioxide (TiO<sub>2</sub>) marker technique is currently widely practiced as a method to evaluate gastrointestinal (GI) passage rate in poultry. However, this method requires sacrificing the animal to obtain digesta samples, is labour-intensive and eliminates the possibility of follow-up studies with the same individual. The aim of this study was to evaluate whether the radiographic methods barium-impregnated polyethylene spheres (BIPS) and barium sulphate (BaSO<sub>4</sub>) suspension are in agreement with the TiO<sub>2</sub> technique and can be used as an alternative method for GI passage rate determination in laying hens. Whole-body radiographs were taken at different time points. Hens of group 3 (<i>n</i> = 55) were orally inoculated with 5 g of feed mixed with 0.15 g TiO<sub>2</sub>, 5 hens per time point sacrificed, and whole gastrointestinal organs (crop, proventriculus, gizzard, small intestine and large intestine) were collected and analysed for TiO<sub>2</sub> content. The average marker passage rate of hens administered BaSO<sub>4</sub> was significantly faster than those administered BIPS (gizzard: 15.2 hr vs. 43.2 hr; small intestine: 15.2 hr vs. 38.4 hr, respectively). A greater percentage of BIPS remained in the crop at 0, 0.5, 2, 3 and 8 hr post-inoculation (p.i.) and in the gizzard at 2, 24, 36 and 48 hr p.i. (all <i>p</i> < 0.05) compared to TiO<sub>2</sub>. In conclusion, the evaluation of the GI transit time is feasible using BIPS, TiO<sub>2</sub> and BaSO<sub>4</sub>. The evaluation of the GI transit time using BIPS and BaSO<sub>4</sub> needs further investigation.
Citation	Journal of Animal Physiology and Animal Nutrition, 103(5), p. 1427-1436
ISSN	1439-0396 0931-2439
Link	https://hdl.handle.net/1959.11/30897
Publisher	Wiley-Blackwell Verlag GmbH
Title	Determination of gastrointestinal passage rate using three different markers in laying hens
Type of document	Journal Article
Entity Type	Publication

Determination of gastrointestinal passage rate using three different markers in laying hens

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