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Title: Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet
Contributor(s): Bond, J J (author); Donaldson, A J (author); Coumans, J V F  (author)orcid ; Austin, K  (author); Ebert, D  (author); Wheeler, D (author); Oddy, V H  (author)orcid 
Publication Date: 2019-01-25
Open Access: Yes
DOI: 10.1186/s40104-019-0314-0
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Abstract: Background: The rumen wall plays a major role in efficient transfer of digested nutrients in the rumen to peripheral tissues through the portal venous system. Some of these substrates are metabolised in the epithelium during this process. To identify the specific proteins involved in these processes, we used proteomic technologies. Protein extracts were prepared from ventral rumen tissue of six sheep fed a fibrous diet at 1.5× maintenance energy requirements. Using a newly developed method, we were able to enzymatically isolate the epithelial cells from underlying tissue layers, thus allowing cytosol and membrane fractions to be independently analysed using liquid chromatography tandem mass spectrometry (LC S/MS).
Results: Using our procedure we identified 570 epithelial proteins in the Ovis aries sequence database. Subcellular locations were largely cytosolic (n = 221) and extracellular (n = 85). However, a quarter of the proteins identified were assigned to the plasma membrane or organelle membranes, some of which transport nutrients and metabolites. Of these 91 were transmembrane proteins (TMHMM), 27 had an N-terminal signal peptide (signalP) and TMHMM motif, 13 had a glycosylphosphatidylinositol (GPI) anchor and signalP sequence, 67 had beta (β) strands or 17 β strands and a transit peptide sequence, indicating the identified proteins were integral or peripheral membrane proteins. Subunits of the 5 protein complexes involved in mitochondrial cellular energy production were well represented. Structural proteins (15%), proteins involved in the metabolism of lipids and proteins (26%) and those with steroid or cytokine action were a feature of the proteome.
Conclusion: Our research has developed a procedure to isolate rumen epithelium proteins from the underlying tissue layers so that they may be profiled using proteomic technologies. The approach improves the number of proteins that can be profiled that are specific to the epithelium of the rumen wall. It provides new insights into the proteins of structural and nutritional importance in the rumen epithelium, that carry out nutrient transport and metabolism, cell growth and signalling.
Publication Type: Journal Article
Source of Publication: Journal of Animal Science and Biotechnology, v.10, p. 1-14
Publisher: BioMed Central Ltd
Place of Publication: United Kingdom
ISSN: 2049-1891
Fields of Research (FoR) 2020: 300199 Agricultural biotechnology not elsewhere classified
Socio-Economic Objective (SEO) 2020: 100199 Environmentally sustainable animal production not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Rural Medicine
School of Science and Technology

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