Author(s) |
Gharib-Naseri, Kosar
Dorigam, Juliano C P
Doranalli, Kiran
Morgan, Natalie
Swick, Robert A
Choct, Mingan
Wu, Shu-Biao
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Publication Date |
2021-03
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Abstract |
Two studies were conducted to investigate the effect of <I>Bacillus amyloliquefaciens</I> CECT 5940 (BA) as a probiotic on growth performance, amino acid digestibility and bacteria population in broiler chickens under a subclinical necrotic enteritis (NE) challenge and/or fed diets with different levels of crude protein (CP). Both studies consisted of a 2 × 2 factorial arrangement of treatments with 480 Ross 308 mix-sexed broiler chickens. In study 1, treatments included 1) NE challenge (+/−), and 2) BA (1.0 × 10<sup>6</sup> CFU/g of feed) supplementation (+/−). In study 2, all birds were under NE challenge, and treatments were 1) CP level (Standard/Reduced [2% less than standard]) and 2) BA (1.0 × 10<sup>6</sup> CFU/g of feed) supplementation (+/−). After inducing NE infection, blood samples were taken on d 16 for uric acid evaluation, and cecal samples were collected for bacterial enumeration. In both studies, ileal digesta was collected on d 35 for nutrient digestibility evaluation. In study 1, the NE challenge reduced body weight gain (BWG), supressed feed conversion ratio (FCR) and serum uric acid levels (<I>P</I> < 0.001). Supplementation of BA increased BWG (<I>P</I> < 0.001) and reduced FCR (<I>P</I> = 0.043) across dietary treatments, regardless of challenge. <I>Bacillus</I> (<I>P</I> = 0.030) and <I>Ruminococcus</I> (<I>P</I> = 0.029) genomic DNA copy numbers and concentration of butyrate (<I>P</I> = 0.017) were higher in birds fed the diets supplemented with BA. In study 2, reduced protein (RCP) diets decreased BWG (<I>P</I> = 0.010) and uric acid levels in serum (<I>P</I> < 0.001). Supplementation of BA improved BWG (<I>P</I> = 0.001) and FCR (<I>P</I> = 0.005) and increased <I>Ruminococcus</I> numbers (<I>P</I> = 0.018) and butyrate concentration (<I>P</I> = 0.033) in the ceca, regardless of dietary CP level. Further, addition of BA reduced <I>Clostridium perfringens</I> numbers only in birds fed with RCP diets (<I>P</I> = 0.039). At d 35, BA supplemented diets showed higher apparent ileal digestibility of cystine (<I>P</I> = 0.013), valine (<I>P</I> = 0.020), and lysine (<I>P</I> = 0.014). In conclusion, this study suggests positive effects of BA supplementation in broiler diets via modulating gut microflora and improving nutrient uptake.
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Citation |
Animal Nutrition, 7(1), p. 185-197
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ISSN |
2405-6383
2405-6545
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Link | |
Publisher |
Zhongguo Xumu Shouyi Xuehui, Chinese Association of Animal Science and Veterinary Medicine
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Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
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Title |
Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge
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Type of document |
Journal Article
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Entity Type |
Publication
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Name | Size | format | Description | Link |
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openpublished/BacillusGharibNaseriMorganSwickChoctWu2021JournalArticle.pdf | 823.509 KB | application/pdf | Published version | View document |