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Title: Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA
Contributor(s): England, A D (author); Kheravii, S K  (author); Musigwa, S  (author)orcid ; Kumar, A  (author); Daneshmand, A (author); Sharma, N K  (author)orcid ; Gharib-Naseri, Kosar  (author)orcid ; Wu, S B  (author)orcid 
Publication Date: 2021-03
Early Online Version: 2020-12-13
Open Access: Yes
DOI: 10.1016/j.psj.2020.12.022
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Abstract: Identification of sex in broiler chickens allows researchers to reduce the level of variation in an experiment caused by the sex effect. Broiler breeds commonly used in research are no longer feather sexable because of the change in their genetics. Other alternate sexing methods are costly and difficult to apply on a large scale. Therefore, a sexing method is required that is both cost effective and highly sensitive as well as having the ability to offer high throughput genotyping. In this study, high-resolution melting (HRM) analysis was used to detect DNA variations present in the gene chromodomain helicase DNA binding 1 protein (CHD1) on the Z and W chromosomes (CHD1Z and CHD1W, respectively) of chickens. In addition, a simplified DNA extraction protocol, which made use of the basal part of chicken feathers, was developed to speed up the sexing procedure. Three pairs of primers, that is, CHD1UNEHRM1F/R, CHD1UNEHRM2F/R, and CHD1UNEHRM3F/R, flanking the polymorphic regions between CHD1Z and CHD1W were used to differentiate male and female chickens via distinct melting curves, typical of homozygous or heterozygous genotypes. The assay was validated by the HRM-sexing of 1,318 broiler chicks and verified by examining the sex of the birds after dissection. This method allows for the sexing of birds within a couple of days, which makes it applicable for use on a large scale such as in nutritional experiments.
Publication Type: Journal Article
Source of Publication: Poultry Science, 100(3), p. 1-9
Publisher: Elsevier BV
Place of Publication: Netherlands
ISSN: 1525-3171
Fields of Research (FoR) 2008: 070204 Animal Nutrition
Fields of Research (FoR) 2020: 300303 Animal nutrition
Socio-Economic Objective (SEO) 2008: 830309 Poultry
Socio-Economic Objective (SEO) 2020: 100411 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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