Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/3047
Title: Functional Dynamics of Human Profilin I and II: Interaction with Phasphatidylinositol (4, 5) Bisphosphate and Poly-L-Proline
Contributor(s): Krishnan, Kannan (author); Moens, Pierre (supervisor)orcid ; Watson, Kenneth (supervisor)
Conferred Date: 2009
Copyright Date: 2009
Open Access: Yes
Handle Link: https://hdl.handle.net/1959.11/3047
Abstract: Profilins are small (~14 kDa) actin binding proteins found in eukaryotes and certain viruses. Profilins are implicated in cytokinesis, membrane trafficking, cell development and cell motility. Apart from actin, profilins also interact with polyphosphoinositides and proline rich domains containing proteins. However, the role of its interaction with Phosphatidylinositol (4, 5) bisphosphate PI(4,5)P₂ is still poorly understood. In this work, the interaction between profilin and PI(4,5)P₂ was investigated using Giant Unilamellar Vesicles (GUV), a model of the cell membrane. Using Number and Brightness analysis, it was demonstrated that, in the absence of profilin, molar ratios of PI(4,5)P₂ above 4% resulted in lipid demixing and cluster formations. Furthermore, adding profilin to GUVs made with 1% PI(4,5)P₂ led to the formation of clusters in the presence of profilin. The diffusion coefficient of PI(4, 5)P₂ measured on GUV membranes showed large variations but the overall diffusion coefficient change, with and without profilin confirmed the cluster formation. Using LAURDAN GP, we found that 16% of PI(4, 5)P₂ molecules on GUVs formed domains that were 2500 nm² in size. When profilin was added, the membranes become more water accessible suggesting differences in the domains in the absence and presence of profilin, however, the domain size remained the same. Phosphorylation of profilin has been previously reported, however, the interaction of phospho-profilin on model membranes has not been studied. Our results with respect to PI(4, 5)P₂ interaction suggested that profilin phosphorylation does not affect its interaction with PI(4, 5)P₂ on the membrane. Furthermore, the cluster formation on GUV membranes was similar to that of wild-type profilin. There are four isoforms of profilin present in humans, apart from the spliced forms of profilin-II. The significance of the presence of these isoforms is not clearly understood. We carried out equilibrium unfolding studies on profilin I and profilin IIA, with and without poly-L-proline. Our results revealed the stabilization effects of poly-L-proline on both profilin isoforms. Our data demonstrated that the stabilization effect of poly-L-proline was greater for profilin-I than profilin-II but that profilin II was intrinsically more stable than profilin I. 1-anilino-8-naphthalene sulphonate fluorescence revealed that there were non-polar binding sites, which are buried in the native state, and become exposed at a low denaturant concentration. These results suggest that profilin binding to proteins containing proline rich domains would stabilize their structure which would have important effects on the dynamics and regulation of profilins function.
Publication Type: Thesis Doctoral
Field of Research Codes: 029901 Biological Physics
Rights Statement: Copyright 2009 - Kannan Krishnan
HERDC Category Description: T2 Thesis - Doctorate by Research
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