Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies

Esmaeili, Atefeh; Knox, Oliver; Juhasz, Albert; Wilson, Susan C

doi:10.1016/j.scitotenv.2020.144783

Author(s)

Esmaeili, Atefeh

Knox, Oliver

Juhasz, Albert

Wilson, Susan C

Publication Date

2021-06-10

Abstract

This study compared chemical extraction methods for the prediction of PAH bioaccumulation in ryegrass (Lolium multiflorum) roots in four Manufactured Gas Plant (MGP) historically (>50 years) contaminated soils. The in-vitro methods compared were butanol (BuOH), non-buffered and buffered 2-hydroxypropyl-β-cyclodextrin extractions (HPCD, Buf-HPCD), potassium persulfate oxidation (KPS), solid phase extraction using Tenax resin (Tenax), and polyoxymethylene solid-phase extraction (POM). Extractions were directly compared with bioaccumulation and modelled using equilibrium partitioning theory (EqPT) with a combination of different partitioning parameters (KOC and KOW values) that aimed to improve predictions. The PAH accumulation in plant roots showed good correlation with concentrations in soils, and higher concentrations of the 4–6 ring PAHs compared with 2–3 ring PAHs. Plant accumulation of 16 PAHs in L. multiflorum was estimated within a factor of 5 using direct comparison for all bioaccessibility extraction methods. Accumulation values predicted using the calculation approach depended on the combination of KOC, KOW parameters and root components (total lipid vs total dry weight) used in calculations. Using KOC values derived from historically contaminated soils improved accuracy of predicted total root accumulation although precision was low. The combined contribution of PAH in lipid and carbohydrate root components (total dry weight) overestimated accumulation and a lipid only approach using generic partitioning parameters provided more accurate and precise approximation of bioaccumulation in roots of L. multiflorum in the soils. Overall, Tenax, POM and HPCD-based extractions showed promising results for predicting L. multiflorum root accumulation using the different approaches. This work significantly extends current knowledge for integrating simple chemical extractions into ecological risk assessment frameworks for the prediction of plant PAH bioavailability in historically contaminated soils.

Citation

Science of the Total Environment, v.772, p. 1-13

ISSN

1879-1026

0048-9697

Pubmed ID

33581513

Link

https://hdl.handle.net/1959.11/30155

Language

en

Publisher

Elsevier BV

Title

Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies

Type of document

Journal Article

Entity Type

Publication

Author(s)	Esmaeili, Atefeh Knox, Oliver Juhasz, Albert Wilson, Susan C
Publication Date	2021-06-10
Abstract	This study compared chemical extraction methods for the prediction of PAH bioaccumulation in ryegrass (<i>Lolium multiflorum</i>) roots in four Manufactured Gas Plant (MGP) historically (>50 years) contaminated soils. The in-vitro methods compared were butanol (BuOH), non-buffered and buffered 2-hydroxypropyl-β-cyclodextrin extractions (HPCD, Buf-HPCD), potassium persulfate oxidation (KPS), solid phase extraction using Tenax resin (Tenax), and polyoxymethylene solid-phase extraction (POM). Extractions were directly compared with bioaccumulation and modelled using equilibrium partitioning theory (EqPT) with a combination of different partitioning parameters (K<sub>OC</sub> and K<sub>OW</sub> values) that aimed to improve predictions. The PAH accumulation in plant roots showed good correlation with concentrations in soils, and higher concentrations of the 4–6 ring PAHs compared with 2–3 ring PAHs. Plant accumulation of 16 PAHs in <i>L. multiflorum</i> was estimated within a factor of 5 using direct comparison for all bioaccessibility extraction methods. Accumulation values predicted using the calculation approach depended on the combination of K<sub>OC</sub>, K<sub>OW</sub> parameters and root components (total lipid vs total dry weight) used in calculations. Using K<sub>OC</sub> values derived from historically contaminated soils improved accuracy of predicted total root accumulation although precision was low. The combined contribution of PAH in lipid and carbohydrate root components (total dry weight) overestimated accumulation and a lipid only approach using generic partitioning parameters provided more accurate and precise approximation of bioaccumulation in roots of <i>L. multiflorum</i> in the soils. Overall, Tenax, POM and HPCD-based extractions showed promising results for predicting <i>L. multiflorum</i> root accumulation using the different approaches. This work significantly extends current knowledge for integrating simple chemical extractions into ecological risk assessment frameworks for the prediction of plant PAH bioavailability in historically contaminated soils.
Citation	Science of the Total Environment, v.772, p. 1-13
ISSN	1879-1026 0048-9697
Pubmed ID	33581513
Link	https://hdl.handle.net/1959.11/30155
Language	en
Publisher	Elsevier BV
Title	Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies
Type of document	Journal Article
Entity Type	Publication

Advancing prediction of polycyclic aromatic hydrocarbon bioaccumulation in plants for historically contaminated soils using Lolium multiflorum and simple chemical in-vitro methodologies

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