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https://hdl.handle.net/1959.11/2998
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DC Field | Value | Language |
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dc.contributor.author | Kitani, Hiroshi | en |
dc.contributor.author | Black, Samuel J. | en |
dc.contributor.author | Nakamura, Yoshio | en |
dc.contributor.author | Naessens, Jan | en |
dc.contributor.author | Murphy, Noel B. | en |
dc.contributor.author | Yokomizo, Yuichi | en |
dc.contributor.author | Gibson, John Paul | en |
dc.contributor.author | Iraqi, Fuad | en |
dc.date.accessioned | 2009-11-10T15:10:00Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Infection and Immunity, 70(4), p. 2210-2214 | en |
dc.identifier.issn | 1098-5522 | en |
dc.identifier.issn | 0019-9567 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/2998 | - |
dc.description.abstract | Mice whose tumor necrosis factor alpha (TNF-α) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with 'Trypanosoma congolense' IL1180 or T. 'brucei brucei' GUTat3.1, confirming the results of earlier studies. To determine whether TNF-α directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF-α on the growth of two isolates of T. 'congolense', IL1180 and IL3338, and two isolates of T. 'brucei brucei', GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF-α used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF-α lots tested, one lot of mouse TNF-α inhibited the growth of both isolates of T. 'brucei brucei' and one lot of bovine TNF-α inhibited the growth of T. 'brucei brucei' ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-α, as well as human TNF-α, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-α/ml of medium). These results suggest that exogenously added recombinant TNF-α generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF-α on 'trypanosome parasitemia' may be indirect, at least with respect to the four strains of trypanosomes reported here. | en |
dc.language | en | en |
dc.publisher | American Society for Microbiology | en |
dc.relation.ispartof | Infection and Immunity | en |
dc.title | Recombinant Tumor Necrosis Factor Alpha Does Not Inhibit The Growth Of African Trypanosomes In Axenic Cultures | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1128/IAI.70.4.2210-2214.2002 | en |
dc.subject.keywords | Animal Breeding | en |
local.contributor.firstname | Hiroshi | en |
local.contributor.firstname | Samuel J. | en |
local.contributor.firstname | Yoshio | en |
local.contributor.firstname | Jan | en |
local.contributor.firstname | Noel B. | en |
local.contributor.firstname | Yuichi | en |
local.contributor.firstname | John Paul | en |
local.contributor.firstname | Fuad | en |
local.subject.for2008 | 070201 Animal Breeding | en |
local.subject.seo2008 | 830399 Livestock Raising not elsewhere classified | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | jgibson5@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | pes:4298 | en |
local.publisher.place | United States of America | en |
local.format.startpage | 2210 | en |
local.format.endpage | 2214 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 70 | en |
local.identifier.issue | 4 | en |
local.contributor.lastname | Kitani | en |
local.contributor.lastname | Black | en |
local.contributor.lastname | Nakamura | en |
local.contributor.lastname | Naessens | en |
local.contributor.lastname | Murphy | en |
local.contributor.lastname | Yokomizo | en |
local.contributor.lastname | Gibson | en |
local.contributor.lastname | Iraqi | en |
dc.identifier.staff | une-id:jgibson5 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:3078 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Recombinant Tumor Necrosis Factor Alpha Does Not Inhibit The Growth Of African Trypanosomes In Axenic Cultures | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Kitani, Hiroshi | en |
local.search.author | Black, Samuel J. | en |
local.search.author | Nakamura, Yoshio | en |
local.search.author | Naessens, Jan | en |
local.search.author | Murphy, Noel B. | en |
local.search.author | Yokomizo, Yuichi | en |
local.search.author | Gibson, John Paul | en |
local.search.author | Iraqi, Fuad | en |
local.uneassociation | Unknown | en |
local.year.published | 2003 | en |
Appears in Collections: | Journal Article |
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