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https://hdl.handle.net/1959.11/2998
Title: | Recombinant Tumor Necrosis Factor Alpha Does Not Inhibit The Growth Of African Trypanosomes In Axenic Cultures | Contributor(s): | Kitani, Hiroshi (author); Black, Samuel J. (author); Nakamura, Yoshio (author); Naessens, Jan (author); Murphy, Noel B. (author); Yokomizo, Yuichi (author); Gibson, John Paul (author); Iraqi, Fuad (author) | Publication Date: | 2003 | DOI: | 10.1128/IAI.70.4.2210-2214.2002 | Handle Link: | https://hdl.handle.net/1959.11/2998 | Abstract: | Mice whose tumor necrosis factor alpha (TNF-α) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with 'Trypanosoma congolense' IL1180 or T. 'brucei brucei' GUTat3.1, confirming the results of earlier studies. To determine whether TNF-α directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF-α on the growth of two isolates of T. 'congolense', IL1180 and IL3338, and two isolates of T. 'brucei brucei', GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF-α used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF-α lots tested, one lot of mouse TNF-α inhibited the growth of both isolates of T. 'brucei brucei' and one lot of bovine TNF-α inhibited the growth of T. 'brucei brucei' ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-α, as well as human TNF-α, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-α/ml of medium). These results suggest that exogenously added recombinant TNF-α generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF-α on 'trypanosome parasitemia' may be indirect, at least with respect to the four strains of trypanosomes reported here. | Publication Type: | Journal Article | Source of Publication: | Infection and Immunity, 70(4), p. 2210-2214 | Publisher: | American Society for Microbiology | Place of Publication: | United States of America | ISSN: | 1098-5522 0019-9567 |
Fields of Research (FoR) 2008: | 070201 Animal Breeding | Socio-Economic Objective (SEO) 2008: | 830399 Livestock Raising not elsewhere classified | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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