Please use this identifier to cite or link to this item:
Title: Recombinant Tumor Necrosis Factor Alpha Does Not Inhibit The Growth Of African Trypanosomes In Axenic Cultures
Contributor(s): Kitani, Hiroshi (author); Black, Samuel J. (author); Nakamura, Yoshio (author); Naessens, Jan (author); Murphy, Noel B. (author); Yokomizo, Yuichi (author); Gibson, John Paul  (author); Iraqi, Fuad (author)
Publication Date: 2003
DOI: 10.1128/IAI.70.4.2210-2214.2002
Handle Link:
Abstract: Mice whose tumor necrosis factor alpha (TNF-α) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with 'Trypanosoma congolense' IL1180 or T. 'brucei brucei' GUTat3.1, confirming the results of earlier studies. To determine whether TNF-α directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF-α on the growth of two isolates of T. 'congolense', IL1180 and IL3338, and two isolates of T. 'brucei brucei', GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF-α used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF-α lots tested, one lot of mouse TNF-α inhibited the growth of both isolates of T. 'brucei brucei' and one lot of bovine TNF-α inhibited the growth of T. 'brucei brucei' ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-α, as well as human TNF-α, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-α/ml of medium). These results suggest that exogenously added recombinant TNF-α generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF-α on 'trypanosome parasitemia' may be indirect, at least with respect to the four strains of trypanosomes reported here.
Publication Type: Journal Article
Source of Publication: Infection and Immunity, 70(4), p. 2210-2214
Publisher: American Society for Microbiology
Place of Publication: United States
ISSN: 0019-9567
Field of Research (FOR): 070201 Animal Breeding
Socio-Economic Objective (SEO): 830399 Livestock Raising not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 252
Views: 253
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
2 files
File Description SizeFormat 
Show full item record


checked on Dec 3, 2018

Page view(s)

checked on Feb 8, 2019
Google Media

Google ScholarTM



Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.