Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/28997
Title: | Use of the rSpaA415 antigen indicates low rates of Erysipelothrix rhusiopathiae infection in farmed cattle from the United States of America and Great Britain |
---|---|
Contributor(s): | Atienzar, Ana I Cubas (author); Gerber, Priscilla F (author)![]() |
Publication Date: | 2019-11-01 |
Open Access: | Yes |
DOI: | 10.1186/s12917-019-2147-7 |
Handle Link: | https://hdl.handle.net/1959.11/28997 |
Abstract: | Background Clinical cases of Erysipelothrix rhusiopathiae, a zoonotic gram-positive bacterium, have been reported in many ruminant species, including in cattle, deer, moose and muskoxen. Fatal cases have been repeatedly reported in cattle over the years but to date there is only one Japanese study investigating the seroprevalence of this bacterium in cattle using the growth agglutination test (GAT). This technique is subjective, time-consuming, expensive and hazardous compared to modern serological tests such as enzyme-linked immunosorbent assays (ELISA) or the newly developed fluorescent microbead-based immunoassays (FMIA). Results The FMIA based on the surface protein SpaA (rSpaA415) antigen of E. rhusiopathiae developed in this study had an almost perfect agreement with the GAT (k = 0.83) and showed a sensitivity of 89.7% and a specificity of 92.9% when compared to the GAT. Overall, detection rates of E. rhusiopathiae antibody positive samples were 13.8% (51/370) in British herds and 6% (12/200) in US herds. Positive cattle were present in 34.3% (24/70) of the investigated British farms and in 34.7% (8/23) of the US farms with an on-farm prevalence of 7.1 to 100% for the British farms and 8.3–30% for the US farms. Conclusions FMIA is a fast, safe and economic alternative to the GAT for the diagnosis of E. rhusiopathiae in cattle. This work is the first seroprevalence study of E. rhusiopathiae in healthy farmed cattle in Great Britain and the US and revealed that infection occurs at a low level. Further investigations to evaluate risks of zoonotic transmission when handling cattle are needed. |
Publication Type: | Journal Article |
Source of Publication: | BMC Veterinary Research, v.15, p. 1-7 |
Publisher: | BioMed Central Ltd |
Place of Publication: | United Kingdom |
ISSN: | 1746-6148 |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology |
Fields of Research (FoR) 2020: | 300914 Veterinary virology |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs |
Socio-Economic Objective (SEO) 2020: | 100410 Pigs |
Peer Reviewed: | Yes |
HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
Appears in Collections: | Journal Article School of Environmental and Rural Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
openpublished/UseOfTherSpaA415Gerber2019JournalArticle.pdf | Published version | 565.38 kB | Adobe PDF Download Adobe | View/Open |
This item is licensed under a Creative Commons License