Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/28219
Title: In vitro versus in situ evaluation of xylan hydrolysis into xylo-oligosaccharides in broiler chicken gastrointestinal tract
Contributor(s): Morgan, Natalie K  (author)orcid ; Wallace, Andrew  (author); Bedford, Michael R (author); Hawking, Kirsten L (author); Rodrigues, Ines  (author); Hilliar, Matthew  (author)orcid ; Choct, Mingan  (author)
Publication Date: 2020-02-15
Early Online Version: 2019-11-19
DOI: 10.1016/j.carbpol.2019.115645
Handle Link: https://hdl.handle.net/1959.11/28219
Abstract: Xylan hydrolysis into xylo-oligosaccharides (XOS) was evaluated both in the gizzard and ileum of broiler chickens, and by a 2-step in vitro digestion assay that simulated the pH, temperature and time period of the gastric and small intestine (SI) phases. Twelve dietary treatments with varying soluble and insoluble xylan levels, either with or without supplemental xylanase, were fed to broiler chickens (n=576) for the in situ analysis, and were exposed to the in vitro assay. Relatedness of the two methods was strong for determination of XOS production in all dietary treatments for X₅, X₄, X₃, X₂ and X₁, respectively, in both the gastric (r=0.980, 0.853, 0.894, 0.870 and 0.951) and small intestine phase (r=0.957, 0.923, 0.940, 0.970, 0.969) (P<0.05). Consequently, the in vitro assay was used to illustrated the diversity of XOS production across different batches of wheat and barley in the presence of xylanase.
Publication Type: Journal Article
Source of Publication: Carbohydrate Polymers, v.230, p. 1-7
Publisher: Elsevier Ltd
Place of Publication: United Kingdom
ISSN: 0144-8617
1879-1344
Field of Research (FOR): 070204 Animal Nutrition
030199 Analytical Chemistry not elsewhere classified
Socio-Economic Objective (SEO): 830309 Poultry
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
860104 Flour Mill and Cereal Food
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Science and Technology

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