In vitro versus in situ evaluation of xylan hydrolysis into xylo-oligosaccharides in broiler chicken gastrointestinal tract

Title
In vitro versus in situ evaluation of xylan hydrolysis into xylo-oligosaccharides in broiler chicken gastrointestinal tract
Publication Date
2020-02-15
Author(s)
Morgan, Natalie K
( author )
OrcID: https://orcid.org/0000-0002-9663-2365
Email: nmorga20@une.edu.au
UNE Id une-id:nmorga20
Wallace, Andrew
Bedford, Michael R
Hawking, Kirsten L
Rodrigues, Ines
Hilliar, Matthew
( author )
OrcID: https://orcid.org/0000-0001-7292-0007
Email: mhillia2@une.edu.au
UNE Id une-id:mhillia2
Choct, Mingan
( author )
OrcID: https://orcid.org/0000-0002-2242-8222
Email: mchoct@une.edu.au
UNE Id une-id:mchoct
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Elsevier Ltd
Place of publication
United Kingdom
DOI
10.1016/j.carbpol.2019.115645
UNE publication id
une:1959.11/28219
Abstract
Xylan hydrolysis into xylo-oligosaccharides (XOS) was evaluated both in the gizzard and ileum of broiler chickens, and by a 2-step in vitro digestion assay that simulated the pH, temperature and time period of the gastric and small intestine (SI) phases. Twelve dietary treatments with varying soluble and insoluble xylan levels, either with or without supplemental xylanase, were fed to broiler chickens (n=576) for the in situ analysis, and were exposed to the in vitro assay. Relatedness of the two methods was strong for determination of XOS production in all dietary treatments for X₅, X₄, X₃, X₂ and X₁, respectively, in both the gastric (r=0.980, 0.853, 0.894, 0.870 and 0.951) and small intestine phase (r=0.957, 0.923, 0.940, 0.970, 0.969) (P<0.05). Consequently, the in vitro assay was used to illustrated the diversity of XOS production across different batches of wheat and barley in the presence of xylanase.
Link
Citation
Carbohydrate Polymers, v.230, p. 1-7
ISSN
1879-1344
0144-8617
Start page
1
End page
7

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