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Title: Tissue distribution, shedding and environmental detection of infectious bursal disease virus genome following infection of meat chickens at two ages
Contributor(s): Jayasundara, JMKGK (author); Walkden-Brown, S W  (author)orcid ; Islam, AFMF  (author); Katz, M E  (author); Renz, K G  (author)
Publication Date: 2018-05
DOI: 10.1111/avj.12691
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Abstract: Objective To compare the effects of infectious bursal disease virus (IBDV) infection of commercial meat chickens at 0 and 16 days old (d.o.) and determine if IBDV vRNA is quantifiable in litter and dust samples.
Methods Ross meat chickens (n = 60) were orally infected or not with IBDV at 0 or 16 d.o. Blood and faecal samples were collected longitudinally to 28 days post infection (dpi) from six chickens and tissues collected weekly from three euthanased chickens. Relative bursal weight was recorded postmortem. IBDV antibody titres in sera were measured using ELISA and VCN was determined in tissues, faeces, litter and dust using qRT-PCR.
Results Chickens infected at 16 d.o. had earlier and more severe bursal atrophy, earlier and higher IBDV vRNA load in lymphoidorgans and an earlier and greater antibody response to infection than those infected at 0 d.o. Faecal shedding of IBDV between 2 and 6 dpi was observed in both groups followed by cessation with the 0 d.o. group and re-initiation of shedding at 28 dpi. IBDV was readily detected and quantified in litter and dust samples.
Conclusions The presence of significant maternal antibody (MAb) titres in 0 d.o. chickens provided protection against IBDV replication and bursal atrophy at 7 and 14 days post infection. The reduced titres of MAb present at 16 d.o. did not prevent rapid IBDV replication and early marked bursal atrophy. The observed resistance of 0 d.o. chickens is likely to be a combination of MAb inhibition of IBDV and true age resistance of neonatal chicks. Measurement of IBDV in litter and dust may have research or diagnostic application.
Publication Type: Journal Article
Source of Publication: Australian Veterinary Journal, 96(5), p. 167-175
Publisher: Wiley-Blackwell Publishing Ltd
Place of Publication: United Kingdom
ISSN: 1751-0813
Fields of Research (FoR) 2008: 070205 Animal Protection (Pests and Pathogens)
070712 Veterinary Virology
Fields of Research (FoR) 2020: 300304 Animal protection (incl. pests and pathogens)
300914 Veterinary virology
Socio-Economic Objective (SEO) 2008: 830309 Poultry
Socio-Economic Objective (SEO) 2020: 100411 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Science and Technology

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