Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/26827
Title: Reliable analysis of volatile compounds from small samples of Eucalyptus magnificata (Myrtaceae)
Contributor(s): Collins, Timothy L  (author); Andrew, Rose L  (author)orcid ; Greatrex, Ben W  (author)orcid ; Bruhl, Jeremy J  (author)orcid 
Publication Date: 2018
Early Online Version: 2018-07-09
DOI: 10.1071/SB18005
Handle Link: https://hdl.handle.net/1959.11/26827
Abstract: Phytochemistry is a source of data for plant systematics. This tool has much more value if herbarium specimens can be used without major damage and if results are comparable with fresh samples. A modified method for the solvent extraction of eucalypt leaf oils for phytochemical analysis and chemotaxonomy studies, including historical herbarium samples by gas chromatography–mass spectrometry (GC–MS), has been statistically assessed using Eucalyptus magnificata L.A.S.Johnson & K.D.Hill leaves. Leaf sample size was reduced by a factor of 250 to minimise damage to herbarium specimens, reduce solvent volume and simplify preparation of solvent extract before analysis. Leaf sampling treatments assessed the effects of the number of leaves and post-harvest air-drying on variation in components in the solvent extract. The results showed no statistically significant effect of leaf mass or the number of leaves used in GC–MS analyses on the precision of the measurements, but a significant difference among treatments for some oil constituents, particularly 1,8-cineole. Most differences in terpenoid concentration were due to variation among plants rather than extraction treatments. Extracts from air-dried herbarium leaves up to 44 years old were directly comparable with those from fresh leaves. Solvent extraction in 2 mL GC–MS vials of ~0.5 cm² (16 mg) of leaf material, using fragments of fresh or air-dried leaves, drastically reduced sample and solvent volumes and showed that sampling from E. magnificata herbarium specimens for chemotaxonomy and chemotyping is a valid method, enabling broader sampling with much lower costs than for traditional fieldwork collections.
Publication Type: Journal Article
Source of Publication: Australian Systematic Botany, 31(3), p. 232-240
Publisher: CSIRO Publishing
Place of Publication: Australia
ISSN: 1030-1887
1446-5701
Field of Research (FOR): 060310 Plant Systematics and Taxonomy
060309 Phylogeny and Comparative Analysis
Socio-Economic Outcome Codes: 961305 Remnant Vegetation and Protected Conservation Areas in Farmland, Arable Cropland and Permanent Cropland Environments
970106 Expanding Knowledge in the Biological Sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Rural Medicine

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