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https://hdl.handle.net/1959.11/26648
Title: | Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing | Contributor(s): | Alghamdi, Othman A (author); King, Nicola (author); Andronicos, Nicholas M (author)![]() ![]() ![]() |
Publication Date: | 2019-02 | Early Online Version: | 2018-07-17 | DOI: | 10.1007/s11010-018-3413-x | Handle Link: | https://hdl.handle.net/1959.11/26648 | Abstract: | Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) and outer medulla membrane vesicles (OMMV) were isolated from the kidneys of young, middle-aged and old rats. The protein expression of PEPT1 was not only increased in BBMV from old rats, but PEPT1 also appeared in OMMV from middle-aged and old rats. SLC15A1 gene expression in the renal cortex increased in middle-aged group. PEPT2 protein expression was not only increased with ageing, but PEPT2 also was found in BBMV from middle-aged and old groups. SLC15A2 gene expression in the renal outer medulla increased in the old group. These changes in the expressions and localisations of PEPT1 and PEPT2 could explain the changes to transport activity in BBMV and OMMV. These findings provide novel insights that would be useful for maintaining protein nutrition and optimising the delivery of some peptidomimetic drugs in elderly individuals. | Publication Type: | Journal Article | Grant Details: | ARC/DP160102063 | Source of Publication: | Molecular and Cellular Biochemistry, 452(1-2), p. 71-82 | Publisher: | Springer New York LLC | Place of Publication: | United States of America | ISSN: | 1573-4919 0300-8177 |
Fields of Research (FoR) 2008: | 110101 Medical Biochemistry: Amino Acids and Metabolites 110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics) |
Fields of Research (FoR) 2020: | 320803 Systems physiology | Socio-Economic Objective (SEO) 2008: | 970111 Expanding Knowledge in the Medical and Health Sciences | Socio-Economic Objective (SEO) 2020: | 200104 Prevention of human diseases and conditions | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Science and Technology |
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