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Title: Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing
Contributor(s): Alghamdi, Othman A (author); King, Nicola  (author); Andronicos, Nicholas M  (author)orcid ; Jones, Graham L  (author)orcid ; Chami, Belal (author); Witting, Paul K (author); Moens, Pierre D J  (author)orcid 
Publication Date: 2019-02
Early Online Version: 2018-07-17
DOI: 10.1007/s11010-018-3413-x
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Abstract: Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) and outer medulla membrane vesicles (OMMV) were isolated from the kidneys of young, middle-aged and old rats. The protein expression of PEPT1 was not only increased in BBMV from old rats, but PEPT1 also appeared in OMMV from middle-aged and old rats. SLC15A1 gene expression in the renal cortex increased in middle-aged group. PEPT2 protein expression was not only increased with ageing, but PEPT2 also was found in BBMV from middle-aged and old groups. SLC15A2 gene expression in the renal outer medulla increased in the old group. These changes in the expressions and localisations of PEPT1 and PEPT2 could explain the changes to transport activity in BBMV and OMMV. These findings provide novel insights that would be useful for maintaining protein nutrition and optimising the delivery of some peptidomimetic drugs in elderly individuals.
Publication Type: Journal Article
Grant Details: ARC/DP160102063
Source of Publication: Molecular and Cellular Biochemistry, 452(1-2), p. 71-82
Publisher: Springer New York LLC
Place of Publication: United States of America
ISSN: 1573-4919
Fields of Research (FoR) 2008: 110101 Medical Biochemistry: Amino Acids and Metabolites
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Fields of Research (FoR) 2020: 320803 Systems physiology
Socio-Economic Objective (SEO) 2008: 970111 Expanding Knowledge in the Medical and Health Sciences
Socio-Economic Objective (SEO) 2020: 200104 Prevention of human diseases and conditions
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Science and Technology

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