To investigate methods for in vitro assessment of anthelmintic efficacy against the chicken nematode Ascaridia galli this study firstly evaluated sample preparation methods including recovery of eggs from excreta using different flotation fluids and induced larval hatching by the deshelling–centrifugation method and the glass-bead method with or without bile. It then evaluated two in vitro assays, the in-ovo larval development assay (LDA) and larval migration inhibition assay (LMIA), for anthelmintic efficacy testing against A. galli using fresh eggs and artificially hatched larvae, respectively. Four anthelmintics, thiabendazole (TBZ), fenbendazole (FBZ), levamisole (LEV) and piperazine (PIP) were employed using an A. galli isolate of known susceptibility. The results suggested that the LDA and LMIA could successfully be used to generate concentration response curves for the tested drugs. The LDA provided EC₅₀ values for inhibition of egg embryonation of 0.084 and 0.071 μg/ml for TBZ and FBZ, respectively. In the LMIA, the values of effective concentration (EC₅₀) of TBZ, FBZ, LEV and PIP were 105.9, 6.32, 349.9 and 6.78 × 10⁷ nM, respectively. For such in vitro studies, a saturated sugar solution showed high egg recovery efficiency (67.8%) and yielded eggs of the highest morphological quality (98.1%) and subsequent developmental ability (93.3%). The larval hatching assays evaluated did not differ in hatching efficiency but the deshelling–centrifugation method yielded larvae that had slightly better survival rates. For final standardization of these tests and establishment of EC₅₀ reference values, tests using isolates of A. galli of defined resistance status need to be performed.

This paper attempts to identify the impact of food wastage on economic growth using the data for 165 countries over the 2014–2018 period. With the help of ordinary least squares (OLS) and generalized linear model (GLM), the study shows that food wastage and poverty impact GDP growth negatively. Poverty and food wastage are positively related. Reducing food wastage can lead to poverty reduction, stimulating GDP growth. Measures are required to reduce food wastage, especially in middle-income countries with a high undernourishment rate. Food wastage reflects poor regulatory capacity, and strengthening the institutional quality can also reduce the wastage of food.

Language and Decolonisation is the first collection to bring together views from across scholarly communities that are committed to the agenda of decolonising knowledge in language study. Edited by leading figures in the field, the chapters offer new insights on how ‘decolonising’ can be adopted as a methodology for charting the next steps in solving practical language-related problems in educational and related social policy areas. Divided into two sections, the book covers the coloniality of language, the materiality of culture and colonial scripts, the decolonisation imperative, multilingualism discourse and decolonisation, and decolonising languages in public discourse. With 20 chapters authored by experts from across the globe, this pioneering collection is an essential reference and resource for advanced students, scholars, and researchers of language and culture, sociolinguistics, decolonial studies, racial studies, and related areas.

Objectives: This study examined (non-)monotonic time trends in psychological and somatic complaints among adolescents, along with gender differences.

Methods: Repeated cross-sectional Health Behaviour in School-aged Children (HBSC) data from 1994 to 2022 covering 15-year-old adolescents from 41 countries (N = 470,797) were analysed. Three polynomial logistic regression models (linear, quadratic, cubic) were tested for best fit, including separate analyses by gender and health complaints dimension.

Results: Time trend patterns varied by gender and health complaints dimension. Increases were found in 82.3% of cases (linear 25%, quadratic U-shaped 28.7%, cubic 28.7%), while 14% showed no clear trend, and 3.7% decreased. Boys typically showed linear increases or no clear trend over time, whereas girls generally showed cubic or U-shaped trends. Psychological complaints often displayed U-shaped or cubic patterns, whereas somatic complaints mostly showed linear increases.

Conclusion: Psychological and somatic complaints demonstrated diverse time trend patterns across countries, with non-monotonic patterns (U-shaped and cubic) frequently observed alongside linear increases. These findings highlight the complexity of changes within countries over three decades, suggesting that linear modelling may not effectively capture this heterogeneity.