Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/2363
Title: The Impact of Some Management Stressors on the Acute Phase Protein Haptoglobin in Beef Cattle
Contributor(s): Slocombe, Lysandra (author); Hinch, Geoffrey  (supervisor)orcid ; Colditz, Ian  (supervisor)
Conferred Date: 2008
Copyright Date: 2006
Open Access: Yes
Handle Link: https://hdl.handle.net/1959.11/2363
Abstract: Assessment of the response of cattle to stressors has previously relied on behavioural changes and physiological responses, in particular cortisol concentrations. This thesis examines the impact of some management practices on the acute phase protein, haptoglobin, in beef cattle. Haptoglobin is readily measured in plasma using a biochemical assay of the peroxidase activity generated by the binding of haptoglobin to haemoglobin. It was found that the accuracy of this method was reduced by haemolysis occurring at the time of blood sampling. The assay presented in this thesis used the peroxidase nature of the haemoglobin-haptoglobin complex in the presence of hydrogen peroxide to catalyse a redox reaction which results in a chromogen changing colour. The assay is highly repeatable (92%) and robust. A correction equation was developed to adjust estimated haptoglobin values for the effect of haemolysis caused at the time of blood collection. The correction equation incorporated measures of plasma haemoglobin concentration and endogenous peroxidase activity present in the sample. Two sites commonly used in cattle for collection of blood samples are the jugular vein and coccygeal vessels. The impact of blood collection site on haematological variables and haptoglobin was examined. Total white blood cell count, lymphocytes, basophils, red blood cells and platelets differed significantly (P<0.05) between the two sites. Basophil counts had a higher concentration in blood collected at the tail. The other variables that significantly differed were lower in the tail vessels compared to the jugular vein. Haptoglobin concentrations did not differ (P>0.05) between blood samples from the two sites. The effect of the production stressors; weaning, transport; intensive management; muscle biopsy and social re-grouping on haptoglobin were analysed.
Publication Type: Thesis Doctoral
Field of Research Codes: 070203 Animal Management
Rights Statement: Copyright 2006 - Lysandra Slocombe
HERDC Category Description: T2 Thesis - Doctorate by Research
Statistics to Oct 2018: Visitors: 59
Views: 78
Downloads: 16
Appears in Collections:Thesis Doctoral

Files in This Item:
8 files
File Description SizeFormat 
open/SOURCE03.pdfAbstract167.74 kBAdobe PDF
Download Adobe
View/Open
open/SOURCE04.pdfThesis1.02 MBAdobe PDF
Download Adobe
View/Open
1 2 Next
Show full item record

Page view(s)

140
checked on Feb 8, 2019

Download(s)

56
checked on Feb 8, 2019
Google Media

Google ScholarTM

Check


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.