Please use this identifier to cite or link to this item:
Title: Production of N2 and N2O from nitrate ingested by sheep
Contributor(s): de Raphelis-Soissan, V (author); Nolan, John V  (author)orcid ; Godwin, Ian R  (author)orcid ; Newbold, J R (author); Eyre, B D (author); Erler, D V (author); Hegarty, Roger  (author)
Publication Date: 2017
DOI: 10.1111/jpn.12725
Handle Link:
Abstract: Supplementing ruminants with nitrate (NO− 3 ) reduces their enteric methane (CH4) emissions; however, the greenhouse gas (GHG) mitigation achieved can be partially offset by small emissions of nitrous oxide (N2O), a more potent GHG. Sheep were dosed intraruminally with 15NO- 3 to investigate whether dietary NO− 3 is a precursor of N2O and/or di-nitrogen gas (N2), and to quantify the amounts of NO− 3 recovered as N2O and N2 in gas emissions from sheep adapted or not adapted to dietary NO− 3. Ruminally cannulated sheep were adapted to a hay diet supplemented with NO− 3 (n = 3; 10 g NO− 3/kg DM) or urea (n = 3; 5.3 g urea/kg DM). On the day of the experiment all sheep were dosed intraruminally with 15NO− 3 and quickly moved into gas-tight chambers to enable recovery of 15N in N2O and N2 to be measured. Measurements of gases accumulating in the chambers were made over 10 successive 50 min periods; this enabled the amount of N2O produced, and the recovery of 15NO− 3-N in N2O and N2 to be determined over a total of 10 hr. Only 0.04% of labelled NO− 3-N was recovered as N2O, and this was not dependent (p > .05) on whether or not the animals had been adapted to dietary NO− 3. Approximatively 3% of 15NO− 3-N was recovered as 15N2, which was also not dependent (p > .05) on whether sheep had been adapted to NO− 3. Because the kinetics of rumen ammonia (NH3) were uncertain, the recovery of 15N from NO− 3 in rumen NH3 could not accurately be quantified, but our results suggest that approximately 76% of dietary NO− 3 was converted to NH3 in the rumen. We conclude that the small amount of NO− 3 recovered in N2 was evidence of denitrification, previously thought not to occur in the rumen.
Publication Type: Journal Article
Source of Publication: Journal of Animal Physiology and Animal Nutrition, 102(1), p. e176-e182
Publisher: Wiley-Blackwell Verlag GmbH
Place of Publication: Germany
ISSN: 0931-2439
Field of Research (FoR) 2008: 070204 Animal Nutrition
Field of Research (FoR) 2020: 300303 Animal nutrition
Socio-Economic Objective (SEO) 2008: 960302 Climate Change Mitigation Strategies
Socio-Economic Objective (SEO) 2020: 190301 Climate change mitigation strategies
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 2
Views: 2
Downloads: 0
Appears in Collections:Journal Article
School of Environmental and Rural Science

Files in This Item:
2 files
File Description SizeFormat 
Show full item record


checked on Oct 18, 2021

Page view(s)

checked on Mar 7, 2019
Google Media

Google ScholarTM





Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.