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|Title:||Production of N2 and N2O from nitrate ingested by sheep||Contributor(s):||de Raphelis-Soissan, V (author); Nolan, John V (author) ; Godwin, Ian R (author) ; Newbold, J R (author); Eyre, B D (author); Erler, D V (author); Hegarty, Roger (author)||Publication Date:||2017||DOI:||10.1111/jpn.12725||Handle Link:||https://hdl.handle.net/1959.11/23272||Abstract:||Supplementing ruminants with nitrate (NO− 3 ) reduces their enteric methane (CH4) emissions; however, the greenhouse gas (GHG) mitigation achieved can be partially offset by small emissions of nitrous oxide (N2O), a more potent GHG. Sheep were dosed intraruminally with 15NO- 3 to investigate whether dietary NO− 3 is a precursor of N2O and/or di-nitrogen gas (N2), and to quantify the amounts of NO− 3 recovered as N2O and N2 in gas emissions from sheep adapted or not adapted to dietary NO− 3. Ruminally cannulated sheep were adapted to a hay diet supplemented with NO− 3 (n = 3; 10 g NO− 3/kg DM) or urea (n = 3; 5.3 g urea/kg DM). On the day of the experiment all sheep were dosed intraruminally with 15NO− 3 and quickly moved into gas-tight chambers to enable recovery of 15N in N2O and N2 to be measured. Measurements of gases accumulating in the chambers were made over 10 successive 50 min periods; this enabled the amount of N2O produced, and the recovery of 15NO− 3-N in N2O and N2 to be determined over a total of 10 hr. Only 0.04% of labelled NO− 3-N was recovered as N2O, and this was not dependent (p > .05) on whether or not the animals had been adapted to dietary NO− 3. Approximatively 3% of 15NO− 3-N was recovered as 15N2, which was also not dependent (p > .05) on whether sheep had been adapted to NO− 3. Because the kinetics of rumen ammonia (NH3) were uncertain, the recovery of 15N from NO− 3 in rumen NH3 could not accurately be quantified, but our results suggest that approximately 76% of dietary NO− 3 was converted to NH3 in the rumen. We conclude that the small amount of NO− 3 recovered in N2 was evidence of denitrification, previously thought not to occur in the rumen.||Publication Type:||Journal Article||Source of Publication:||Journal of Animal Physiology and Animal Nutrition, 102(1), p. e176-e182||Publisher:||Wiley-Blackwell Verlag GmbH||Place of Publication:||Germany||ISSN:||0931-2439
|Field of Research (FOR):||070204 Animal Nutrition||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 2
|Appears in Collections:||Journal Article|
School of Environmental and Rural Science
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