Coumans-Moens, Joelle; Yeoh, Thomas; Seeto, Reginald K; Keogh, Anne; Brennan, Karen; Gunning, Peter; Hardeman, Edna; dos Remedios, Cristobal G

Author(s)

Coumans-Moens, Joelle

Yeoh, Thomas

Seeto, Reginald K

Keogh, Anne

Brennan, Karen

Gunning, Peter

Hardeman, Edna

dos Remedios, Cristobal G

Publication Date

1997

Abstract

This paper examines the quantitative relationship between the expression of myosin heavy chain (MHC) and actin at both the levels of their mRNAs and their proteins. Explanted human left ventricle tissues were obtained from non-diseased (ND) individuals and from dilated cardiomyopathy (DCM) patients with terminally failing hearts who underwent heart transplantation. We found: (1) there are substantial differences in the stoichiometry of sarcomeric MHC and actin transcripts in hearts of DCM patients as well as in ND individuals; (2) there are substantial differences between levels of total sarcomeric actin transcripts from different individual patients; (3) by and large variations in transcript levels between samples from the same heart are much less than between samples from different hearts; and (4) the ratio of MHC to sarcomeric actin proteins expressed by different ND and DCM hearts remains essentiallly constant. We conclude that the human ventricle can accomodate a substantial imbalance between sarcomeric MHC and actin mRNA levels while maintaining a constant ratio of their corresponding proteins.

Citation

Journal of Molecular and Cellular Cardiology, 29(3), p. 895-905

ISSN

1095-8584

0022-2828

Link

https://hdl.handle.net/1959.11/22931

Publisher

Academic Press

Title

Variations in the Relative mRNA Levels of Actins and Myosin Heavy Chains do not Produce Corresponding Differences in their Proteins in the Adult Human Heart

Type of document

Journal Article

Entity Type

Publication

Author(s)	Coumans-Moens, Joelle Yeoh, Thomas Seeto, Reginald K Keogh, Anne Brennan, Karen Gunning, Peter Hardeman, Edna dos Remedios, Cristobal G
Publication Date	1997
Abstract	This paper examines the quantitative relationship between the expression of myosin heavy chain (MHC) and actin at both the levels of their mRNAs and their proteins. Explanted human left ventricle tissues were obtained from non-diseased (ND) individuals and from dilated cardiomyopathy (DCM) patients with terminally failing hearts who underwent heart transplantation. We found: (1) there are substantial differences in the stoichiometry of sarcomeric MHC and actin transcripts in hearts of DCM patients as well as in ND individuals; (2) there are substantial differences between levels of total sarcomeric actin transcripts from different individual patients; (3) by and large variations in transcript levels between samples from the same heart are much less than between samples from different hearts; and (4) the ratio of MHC to sarcomeric actin proteins expressed by different ND and DCM hearts remains essentiallly constant. We conclude that the human ventricle can accomodate a substantial imbalance between sarcomeric MHC and actin mRNA levels while maintaining a constant ratio of their corresponding proteins.
Citation	Journal of Molecular and Cellular Cardiology, 29(3), p. 895-905
ISSN	1095-8584 0022-2828
Link	https://hdl.handle.net/1959.11/22931
Publisher	Academic Press
Title	Variations in the Relative mRNA Levels of Actins and Myosin Heavy Chains do not Produce Corresponding Differences in their Proteins in the Adult Human Heart
Type of document	Journal Article
Entity Type	Publication

Variations in the Relative mRNA Levels of Actins and Myosin Heavy Chains do not Produce Corresponding Differences in their Proteins in the Adult Human Heart

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