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https://hdl.handle.net/1959.11/22831
Title: | Olive (Olea europaea L.) Biophenols: A Nutriceutical against Oxidative Stress in SH-SY5Y Cells | Contributor(s): | Omar, Syed Haris (author); Kerr, Philip G (author); Scott, Christopher J (author); Hamlin, Adam (author) ; Obied, Hassan K (author) | Publication Date: | 2017 | Open Access: | Yes | DOI: | 10.3390/molecules22111858 | Handle Link: | https://hdl.handle.net/1959.11/22831 | Abstract: | Plant biophenols have been shown to be effective in the modulation of Alzheimer's disease (AD) pathology resulting from free radical-induced oxidative stress and imbalance of the redox chemistry of transition metal ions (e.g., iron and copper). On the basis of earlier reported pharmacological activities, olive biophenols would also be expected to have anti-Alzheimer's activity. In the present study, the antioxidant activity of individual olive biophenols (viz. caffeic acid, hydroxytyrosol, oleuropein, verbascoside, quercetin, rutin and luteolin) were evaluated using superoxide radical scavenging activity (SOR), hydrogen peroxide (H2O2) scavenging activity, and ferric reducing ability of plasma (FRAP) assays. The identification and antioxidant activities in four commercial olive extracts-Olive leaf extractTM (OLE), Olive fruit extractTM (OFE), Hydroxytyrosol ExtremeTM (HTE), and Olivenol plusTM (OLP)-were evaluated using an on-line HPLC-ABTS•+ assay, and HPLC-DAD-MS analysis. Oleuropein and hydroxytyrosol were the predominant biophenols in all the extracts. Among the single compounds examined, quercetin (EC50: 93.97 μM) and verbascoside (EC50: 0.66 mM) were the most potent SOR and H2O2 scavengers respectively. However, OLE and HTE were the highest SOR (EC50: 1.89 μg/mL) and H2O2 (EC50: 115.8 μg/mL) scavengers among the biophenol extracts. The neuroprotection of the biophenols was evaluated against H2O2-induced oxidative stress and copper (Cu)-induced toxicity in neuroblastoma (SH-SY5Y) cells. The highest neuroprotection values (98% and 92%) against H2O2-induced and Cu-induced toxicities were shown by the commercial extract HTETM. These were followed by the individual biophenols, caffeic acid (77% and 64%) and verbascoside (71% and 72%). Our results suggest that olive biophenols potentially serve as agents for the prevention of neurodegenerative diseases such as AD, and other neurodegenerative ailments that are caused by oxidative stress. | Publication Type: | Journal Article | Source of Publication: | Molecules, 22(11), p. 1-20 | Publisher: | MDPI AG | Place of Publication: | Switzerland | ISSN: | 1420-3049 1431-5165 |
Fields of Research (FoR) 2008: | 110904 Neurology and Neuromuscular Diseases | Fields of Research (FoR) 2020: | 310101 Analytical biochemistry 320903 Central nervous system |
Socio-Economic Objective (SEO) 2008: | 970111 Expanding Knowledge in the Medical and Health Sciences | Socio-Economic Objective (SEO) 2020: | 280114 Expanding knowledge in Indigenous studies 280103 Expanding knowledge in the biomedical and clinical science |
Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Science and Technology |
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