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https://hdl.handle.net/1959.11/22392
Title: | Evaluation of the efficacy of a commercial inactivated genogroup 2b-based porcine epidemic diarrhea virus (PEDV) vaccine and experimental live genogroup 1b exposure against 2b challenge | Contributor(s): | Opriessnig, Tanja (author); Gerber, Priscilla Freitas (author) ; Shen, Huigang (author); De Castro, Alessandra Marnie M G (author); Zhang, Jianqiang (author); Chen, Qi (author); Halbur, Patrick (author) | Publication Date: | 2017 | Open Access: | Yes | DOI: | 10.1186/s13567-017-0472-z | Handle Link: | https://hdl.handle.net/1959.11/22392 | Abstract: | Porcine epidemic diarrhea virus strains from the G1b cluster are considered less pathogenic compared to the G2b cluster. The aim of this study was to compare the ability of G1b-based live virus exposure against use of a commercial G2b–based inactivated vaccine to protect growing pigs against G2b challenge. Thirty-nine PEDV naïve pigs were randomly divided into five groups: EXP-IM-1b (intramuscular G1b exposure; G2b challenge), EXP-ORAL-1b (oral G1b exposure; G2b challenge), VAC-IM-2b (intramuscular commercial inactivated G2b vaccination; G2b challenge), POS-CONTROL (sham-vaccination; G2b challenge) and NEG-CONTROL (sham-vaccination; sham-challenge). Pigs were vaccinated/exposed at 3 weeks of age (day post-vaccination 0, dpv 0), VAC-IM-2b pigs were revaccinated at dpv 14, and the pigs were challenged at dpv 28. Among all groups, VAC-IM-2b pigs had significantly higher anti-PEDV IgG levels on dpv 21 and 28 while EXP-ORAL-1b pigs had significantly higher anti-PEDV IgA levels on dpv 14, 21, 28 and 35. EXP-ORAL-1b also had detectable IgA in feces. Intramuscular PEDV exposure did not result in a detectable antibody response in EXP-IM-1b pigs. The fecal PEDV RNA levels in VAC-IM-2b pigs were significantly lower 5–7 days after challenge compared to the POS-CONTROL group. Under the study conditions a commercial inactivated G2b-based vaccine protected pigs against G2b challenge, as evidenced by reduction of PEDV RNA in feces for 3–4 logs during peak shedding and a shorter viral shedding duration. The oral, but not the intramuscular, experimental G1b-based live virus exposure induced a high anti-PEDV IgA response prior to challenge, which apparently did not impact PEDV shedding compared to POS-CONTROL pigs. | Publication Type: | Journal Article | Source of Publication: | Veterinary Research, v.48, p. 1-10 | Publisher: | BioMed Central Ltd | Place of Publication: | United Kingdom | ISSN: | 1297-9716 0928-4249 |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070706 Veterinary Medicine |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300907 Veterinary medicine (excl. urology) |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Environmental and Rural Science |
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