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Title: Pathogenicity, tissue distribution, shedding and environmental detection of two strains of IBDV following infection of chickens at 0 and 14 days of age
Contributor(s): Jayasundara, Kanchana (author); Walkden-Brown, Steve W  (author)orcid ; Katz, Margaret  (author); Islam, Fakhrul  (author); Renz, Katrin  (author); McNally, J (author); Hunt, Peter William (author)
Publication Date: 2017
DOI: 10.1080/03079457.2016.1248898
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Abstract: Infectious bursal disease virus (IBDV) is endemic to most poultry-producing countries worldwide. Immunosuppressive classical and variant IBDV strains endemic to Australia are genetically distinct from other international strains. We report the results of infection experiments with Australian classical strain 06/95 and variant strain 02/95 in SPF chickens. We tested the effects of strain and age of infection on bursal atrophy, viral RNA (vRNA) load in bursa of Fabricius (bursa), spleen, thymus, caecal tonsils, faeces, litter and exhaust dust as determined by real-time reverse transcriptase polymerase chain reaction. The two IBDV strains did not differ in the degree of bursal atrophy induced, lymphoid organ distribution and faecal shedding but variant strain 02/95 induced a greater antibody response to the infection than classical strain 06/95 which was associated with a more rapid decline in IBDV vRNA genome copy number (VCN) in lymphoid organs and faeces. Infection at 14 days of age induced greater bursal atrophy and higher vRNA copy number in lymphoid tissues than infection on the day of hatching, indicating true age susceptibility independent of maternal antibody (Mab) status. The direction of the association between rankings for IBDV vRNA load in bursa and relative bursal weight changed from positive at 3 and 6 days post-infection to negative at 28 days post-infection. Intra-tracheal administration of dust collected from chickens infected with IBDV resulted in successful transmission of IBDV. IBDV vRNA was detected successfully at high levels in the environmental litter and dust samples.
Publication Type: Journal Article
Source of Publication: Avian Pathology, 46(3), p. 242-255
Publisher: Taylor & Francis
Place of Publication: United Kingdom
ISSN: 1465-3338
Fields of Research (FoR) 2008: 070712 Veterinary Virology
070205 Animal Protection (Pests and Pathogens)
070703 Veterinary Diagnosis and Diagnostics
Fields of Research (FoR) 2020: 300914 Veterinary virology
300304 Animal protection (incl. pests and pathogens)
300904 Veterinary diagnosis and diagnostics
Socio-Economic Objective (SEO) 2008: 830309 Poultry
Socio-Economic Objective (SEO) 2020: 100411 Poultry
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Science and Technology

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