Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/22314
Title: Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest
Contributor(s): Keeling, Christopher I (author); Yuen, Macaire M S (author); Henderson, Hannah (author); Janes, Jasmine  (author)orcid ; Zhao, Yongjun (author); Pandoh, Pawan (author); Moore, Richard (author); Sperling, Felix A H (author); Huber, Dezene P W (author); Birol, Inanc (author); Jones, Steven J M (author); Bohlmann, Joerg (author); Liao, Nancy Y (author); Docking, T Roderick (author); Chan, Simon K (author); Taylor, Greg A (author); Palmquist, Diana L (author); Jackman, Shaun D (author); Nguyen, Anh (author); Li, Maria (author)
Publication Date: 2013
Open Access: Yes
DOI: 10.1186/gb-2013-14-3-r27Open Access Link
Handle Link: https://hdl.handle.net/1959.11/22314
Abstract: Background: The mountain pine beetle, 'Dendroctonus ponderosae' Hopkins, is the most serious insect pest of western North American pine forests. A recent outbreak destroyed more than 15 million hectares of pine forests, with major environmental effects on forest health, and economic effects on the forest industry. The outbreak has in part been driven by climate change, and will contribute to increased carbon emissions through decaying forests. Results: We developed a genome sequence resource for the mountain pine beetle to better understand the unique aspects of this insect's biology. A draft 'de novo' genome sequence was assembled from paired-end, shortread sequences from an individual field-collected male pupa, and scaffolded using mate-paired, short-read genomic sequences from pooled field-collected pupae, paired-end short-insert whole-transcriptome shotgun sequencing reads of mRNA from adult beetle tissues, and paired-end Sanger EST sequences from various life stages. We describe the cytochrome P450, glutathione S-transferase, and plant cell wall-degrading enzyme gene families important to the survival of the mountain pine beetle in its harsh and nutrient-poor host environment, and examine genome-wide single-nucleotide polymorphism variation. A horizontally transferred bacterial sucrose-6- phosphate hydrolase was evident in the genome, and its tissue-specific transcription suggests a functional role for this beetle. Conclusions: Despite Coleoptera being the largest insect order with over 400,000 described species, including many agricultural and forest pest species, this is only the second genome sequence reported in Coleoptera, and will provide an important resource for the Curculionoidea and other insects.
Publication Type: Journal Article
Source of Publication: Genome Biology, v.14, p. 1-19
Publisher: BioMed Central Ltd
Place of Publication: United Kingdom
ISSN: 1474-760X
1474-7596
Fields of Research (FoR) 2008: 060399 Evolutionary Biology not elsewhere classified
060306 Evolutionary Impacts of Climate Change
Fields of Research (FoR) 2020: 310499 Evolutionary biology not elsewhere classified
310406 Evolutionary impacts of climate change
Socio-Economic Objective (SEO) 2008: 960399 Climate and Climate Change not elsewhere classified
960404 Control of Animal Pests, Diseases and Exotic Species in Forest and Woodlands Environments
Socio-Economic Objective (SEO) 2020: 180602 Control of pests, diseases and exotic species in terrestrial environments
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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