Emissions of volatile odorous metabolites by 'Clostridium perfringens' - in vitro study using two broth cultures

Author(s)
Sharma, Nishchal Kumar
Keerqin, Chake
Wu, Shubiao
Choct, Mingan
Swick, Robert A
Publication Date
2017
Abstract
An in vitro study was carried out to investigate the volatile odorous metabolites produced by necrotic enteritis inducing 'Clostridium perfringens' (Cp) type A field strain (EHE-NE18) cultured in two broth media (thioglycollate broth, brain heart infusion broth) at three levels (0, 103 cfu/mL, 106 cfu/mL) using 2 x 3 factorial arrangement of treatments, with each replicated three times. The culture media with and without Cp was freshly prepared on the day of measurement and the culture headspace concentration of odorants was measured using selected ion-flow tube mass spectrometry (SIFT-MS). The results showed that media type and Cp level affected the concentration of a range of volatile odorous metabolites including sulfur compounds, alcohols, ketones, amines, and carboxylic acids. Thus, Cp contributes to the production of a wide range of odorous metabolites that can impart noxious smell during infection.
Citation
Poultry Science, 96(9), p. 3291-3297
ISSN
1525-3171
0032-5791
Link
Language
en
Publisher
Oxford University Press
Title
Emissions of volatile odorous metabolites by 'Clostridium perfringens' - in vitro study using two broth cultures
Type of document
Journal Article
Entity Type
Publication

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