Immune responses following experimental infection with 'Ascaridia galli' and necrotic enteritis in broiler chickens

Title
Immune responses following experimental infection with 'Ascaridia galli' and necrotic enteritis in broiler chickens
Publication Date
2017
Author(s)
Ruhnke, Isabelle
( author )
OrcID: https://orcid.org/0000-0001-5423-9306
Email: iruhnke@une.edu.au
UNE Id une-id:iruhnke
Andronicos, Nicholas
( author )
OrcID: https://orcid.org/0000-0001-5881-2296
Email: nandroni@une.edu.au
UNE Id une-id:nandroni
Swick, Robert A
( author )
OrcID: https://orcid.org/0000-0003-3376-1677
Email: rswick@une.edu.au
UNE Id une-id:rswick
Hine, Brad C
Sharma, Nisha
Kheravii, Sarbast K
Wu, Shubiao
( author )
OrcID: https://orcid.org/0000-0002-1790-6015
Email: swu3@une.edu.au
UNE Id une-id:swu3
Hunt, Peter
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Taylor & Francis
Place of publication
United Kingdom
DOI
10.1080/03079457.2017.1330536
UNE publication id
une:22470
Abstract
Broilers commonly suffer from necrotic enteritis (NE). Other gastrointestinal infectious diseases affect poultry, including nematode infections which are considered a re-emerging disease in barn and free-range systems. The aim of this study was to characterise the immune response of broilers after artificial infection with NE and contrast these with responses to the nematode 'Ascaridia galli' and determine whether immune parameters measured during the course of infection can be used to distinguish infected from uninfected birds. A total of 96 one-day-old male Ross 308 broiler chickens were used in this study. At 10 days of age, broilers were randomly assigned to one of the following treatment groups: control birds (n = 32), 'A. galli' infected birds (n = 32), or necrotic enteritis infected birds (NE; n = 32) and inoculated with the appropriate infective agents. The immune response of birds was monitored through evaluation of haematology parameters, acute phase protein production, and intraepithelial intestinal lymphocyte population changes at 11, 16, 20 and 32 days of age. T-helper cells (CD4+CD8-) increased significantly over time, and were significantly higher in 'A. galli' and NE compared to day 10 controls. In conclusion, α-1 glycoprotein levels can distinguish birds with NE from other birds, inlcuding those infected with 'A. galli', also T-helper cell numbers can distinguish both NE and 'A. galli' from uninfected birds and thirdly, 10 days post infection is the best time point to evaluate the bird's immune response for 'A. galli' infections.
Link
Citation
Avian Pathology, 46(6), p. 602-609
ISSN
1465-3338
0307-9457
Start page
602
End page
609
Rights
Attribution-NonCommercial 4.0 International

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