Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/22203
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dc.contributor.authorSamiullah, Samiullahen
dc.contributor.authorRoberts, Julieten
dc.contributor.authorWu, Shubiaoen
dc.date.accessioned2017-11-30T20:18:00Z
dc.date.issued2017en
dc.identifier.citationScientific Reports, v.7, p. 1-13en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/1959.11/22203en
dc.description.abstractShell colour is an important trait for eggs and an understanding of pigment deposition will assist potential management of egg shell colour loss. We demonstrated that nicarbazin feeding downregulated ALAS1 and reduced protoporphyrin IX (PP IX) in both shell gland and eggshell, indicating the role of nicarbazin in inhibiting the synthesis of PP IX. Additionally, the expression levels of the genes did not show sequential upregulation in the same order of diurnal time-points (TP) during egg formation. The gene SLC25A38, responsible for transporting glycine from cytoplasm to mitochondria, and the gene ALAS1, encoding rate-limiting enzyme (delta-aminolevulinic acid synthase 1), had higher expression at 15 hr, as compared with 2, 5 and 23.5 hrs postoviposition. Interestingly, ABCB6, a gene encoding an enzyme responsible for transporting coproporphyrinogen III, showed higher expression level at 2 and 5 hrs. However, the expression of CPOX that converts coproporphyrinogen III to protoporphyrinogen III, and ABCG2 that transports PP IX out from mitochondria did not alter. Nevertheless, mitochondrial count per cell did not show consistent change in response to time-points postoviposition and nicarbazin feeding. The information obtained in the study sheds light on how nicarbazin disrupts the synthesis of PP IX.en
dc.languageenen
dc.publisherNature Publishing Groupen
dc.relation.ispartofScientific Reportsen
dc.titleDownregulation of 'ALAS1' by nicarbazin treatment underlies the reduced synthesis of protoporphyrin IX in shell gland of laying hensen
dc.typeJournal Articleen
dc.identifier.doi10.1038/s41598-017-06527-yen
dcterms.accessRightsGolden
dc.subject.keywordsBiological Sciencesen
local.contributor.firstnameSamiullahen
local.contributor.firstnameJulieten
local.contributor.firstnameShubiaoen
local.subject.for2008069999 Biological Sciences not elsewhere classifieden
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of E&RS - Animal Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailsamiull2@une.edu.auen
local.profile.emailswu3@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-chute-20171115-084651en
local.publisher.placeUnited Kingdomen
local.identifier.runningnumber6253en
local.format.startpage1en
local.format.endpage13en
local.peerreviewedYesen
local.identifier.volume7en
local.access.fulltextYesen
local.contributor.lastnameSamiullahen
local.contributor.lastnameRobertsen
local.contributor.lastnameWuen
dc.identifier.staffune-id:samiull2en
dc.identifier.staffune-id:swu3en
local.profile.orcid0000-0002-1790-6015en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:22393en
local.identifier.handlehttps://hdl.handle.net/1959.11/22203en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleDownregulation of 'ALAS1' by nicarbazin treatment underlies the reduced synthesis of protoporphyrin IX in shell gland of laying hensen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.description.statisticsepubsVisitors: 13<br />Views: 17<br />Downloads: 2en
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