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|Title:||Downregulation of 'ALAS1' by nicarbazin treatment underlies the reduced synthesis of protoporphyrin IX in shell gland of laying hens||Contributor(s):||Samiullah, Samiullah (author); Roberts, Juliet (author); Wu, Shubiao (author)||Publication Date:||2017||Open Access:||Yes||DOI:||10.1038/s41598-017-06527-y||Handle Link:||https://hdl.handle.net/1959.11/22203||Abstract:||Shell colour is an important trait for eggs and an understanding of pigment deposition will assist potential management of egg shell colour loss. We demonstrated that nicarbazin feeding downregulated ALAS1 and reduced protoporphyrin IX (PP IX) in both shell gland and eggshell, indicating the role of nicarbazin in inhibiting the synthesis of PP IX. Additionally, the expression levels of the genes did not show sequential upregulation in the same order of diurnal time-points (TP) during egg formation. The gene SLC25A38, responsible for transporting glycine from cytoplasm to mitochondria, and the gene ALAS1, encoding rate-limiting enzyme (delta-aminolevulinic acid synthase 1), had higher expression at 15 hr, as compared with 2, 5 and 23.5 hrs postoviposition. Interestingly, ABCB6, a gene encoding an enzyme responsible for transporting coproporphyrinogen III, showed higher expression level at 2 and 5 hrs. However, the expression of CPOX that converts coproporphyrinogen III to protoporphyrinogen III, and ABCG2 that transports PP IX out from mitochondria did not alter. Nevertheless, mitochondrial count per cell did not show consistent change in response to time-points postoviposition and nicarbazin feeding. The information obtained in the study sheds light on how nicarbazin disrupts the synthesis of PP IX.||Publication Type:||Journal Article||Source of Publication:||Scientific Reports, v.7, p. 1-13||Publisher:||Nature Publishing Group||Place of Publication:||United Kingdom||ISSN:||2045-2322||Field of Research (FOR):||069999 Biological Sciences not elsewhere classified||Socio-Economic Outcome Codes:||970106 Expanding Knowledge in the Biological Sciences||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 13
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