Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus

Title
Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus
Publication Date
2015
Author(s)
Adu-Gyamfi, Emmanuel
Johnson, Kristen A
Fraser, Mark E
Scott, Jordan L
Soni, Smita P
Jones, Keaton R
Gratton, Enrico
Tessier, Charles R
Stahelina, Robert V
Digman, Michelle
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
American Society for Microbiology
Place of publication
United States of America
DOI
10.1128/JVI.01087-15
UNE publication id
une:22290
Abstract
Lipid-enveloped viruses replicate and bud from the host cell where they acquire their lipid coat. Ebola virus, which buds from the plasma membrane of the host cell, causes viral hemorrhagic fever and has a high fatality rate. To date, little has been known about how budding and egress of Ebola virus are mediated at the plasma membrane. We have found that the lipid phosphatidylserine (PS) regulates the assembly of Ebola virus matrix protein VP40. VP40 binds PS-containing membranes with nanomolar affinity, and binding of PS regulates VP40 localization and oligomerization on the plasma membrane inner leaflet. Further, alteration of PS levels in mammalian cells inhibits assembly and egress of VP40. Notably, interactions of VP40 with the plasma membrane induced exposure of PS on the outer leaflet of the plasma membrane at sites of egress, whereas PS is typically found only on the inner leaflet. Taking the data together, we present a model accounting for the role of plasma membrane PS in assembly of Ebola virus-like particles.
Link
Citation
Journal of Virology, 89(18), p. 9440-9453
ISSN
1098-5514
Start page
9440
End page
9453

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