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Title: Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus
Contributor(s): Adu-Gyamfi, Emmanuel (author); Johnson, Kristen A (author); Fraser, Mark E (author); Scott, Jordan L (author); Soni, Smita P (author); Jones, Keaton R (author); Gratton, Enrico (author); Tessier, Charles R (author); Stahelina, Robert V (author); Digman, Michelle  (author)
Publication Date: 2015
Open Access: Yes
DOI: 10.1128/JVI.01087-15Open Access Link
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Abstract: Lipid-enveloped viruses replicate and bud from the host cell where they acquire their lipid coat. Ebola virus, which buds from the plasma membrane of the host cell, causes viral hemorrhagic fever and has a high fatality rate. To date, little has been known about how budding and egress of Ebola virus are mediated at the plasma membrane. We have found that the lipid phosphatidylserine (PS) regulates the assembly of Ebola virus matrix protein VP40. VP40 binds PS-containing membranes with nanomolar affinity, and binding of PS regulates VP40 localization and oligomerization on the plasma membrane inner leaflet. Further, alteration of PS levels in mammalian cells inhibits assembly and egress of VP40. Notably, interactions of VP40 with the plasma membrane induced exposure of PS on the outer leaflet of the plasma membrane at sites of egress, whereas PS is typically found only on the inner leaflet. Taking the data together, we present a model accounting for the role of plasma membrane PS in assembly of Ebola virus-like particles.
Publication Type: Journal Article
Source of Publication: Journal of Virology, 89(18), p. 9440-9453
Publisher: American Society for Microbiology
Place of Publication: United States of America
ISSN: 1098-5514
Field of Research (FOR): 029901 Biological Physics
111601 Cell Physiology
060199 Biochemistry and Cell Biology not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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