Managing the rumen to limit the incidence and severity of nitrite poisoning in nitrate-supplemented ruminants

Abstract

Inclusion of nitrate (NO₃⁻) in ruminant diets is a means of increasing non-protein nitrogen intake while at the same time reducing emissions of enteric methane (CH₄) and, in Australia, gaining carbon credits. Rumen microorganisms contain intracellular enzymes that use hydrogen (H₂) released during fermentation to reduce NO₃⁻ to nitrite (NO₂⁻), and then reduce the resulting NO₂⁻ to ammonia or gaseous intermediates such as nitrous oxide (N₂O) and nitric oxide (NO). This diversion of H2 reduces CH₄ formation in the rumen. If NO₂⁻ accumulates in the rumen, it may inhibit growth of methanogens and other microorganisms and this may further reduce CH4 production, but also lower feed digestibility. If NO₂⁻ is absorbed and enters red blood cells, methaemoglobin is formed and this lowers the oxygen-carrying capacity of the blood. Nitric oxide produced from absorbed NO₂⁻ reduces blood pressure, which, together with the effects of methaemoglobin, can, at times, lead to extreme hypoxia and death. Nitric oxide, which can be formed in the gut as well as in tissues, has a variety of physiological effects, e.g. it reduces primary rumen contractions and slows passage of digesta, potentially limiting feed intake. It is important to find management strategies that minimise the accumulation of NO₂⁻; these include slowing the rate of presentation of NO₃⁻ to rumen microbes or increasing the rate of removal of NO₂⁻, or both. The rate of reduction of NO₃⁻ to NO₂⁻ depends on the level of NO₃⁻ in feed and its ingestion rate, which is related to the animal's feeding behaviour. After NO₃⁻ is ingested, its peak concentration in the rumen depends on its rate of solubilisation. Once in solution, NO₃⁻ is imported by bacteria and protozoa and quickly reduced to NO₂⁻. One management option is to encapsulate the NO₃⁻ supplement to lower its solubility. Acclimating animals to NO₃⁻ is an established management strategy that appears to limit NO₂⁻ accumulation in the rumen by increasing microbial nitrite reductase activity more than nitrate reductase activity; however, it does not guarantee complete protection from NO₂⁻ poisoning. Adding concentrates into nitrate-containing diets also helps reduce the risk of poisoning and inclusion of microbial cultures with enhanced NO₂⁻ - reducing properties is another potential management option. A further possibility is to inhibit NO₂⁻ absorption. Animals differ in their tolerance to NO₃⁻ supplementation, so there may be opportunities for breeding animals more tolerant of dietary NO₃⁻. Our review aims to integrate current knowledge of microbial processes responsible for accumulation of NO₂⁻ in rumen fluid and to identify management options that could minimise the risks of NO₂⁻ poisoning while reducing methane emissions and maintaining or enhancing livestock production.