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Title: The effects of chronic nitrate supplementation on erythrocytic methaemoglobin reduction in cattle
Contributor(s): Godwin, Ian (author); Li, Li (author); Luijben, K (author); Oelbrandt, N (author); Velazco, J I (author); Miller, J (author); Hegarty, R (author)
Publication Date: 2015
DOI: 10.1071/an13366
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Abstract: Calcium nitrate and urea were fed as a supplement on an isonitrogenous basis to Angus steers and their erythrocytic methaemoglobin concentrations and NADH- and NADPH-methaemoglobin reductase levels were measured over a 54-day period. Methaemoglobin concentrations remained elevated despite increases in NADH-methaemoglobin reductase activity. In a second experiment, Brahman cross steers were fed either calcium nitrate or urea supplements for 111 days. Blood cells were then taken, washed and exposed to sodium nitrite to convert all haemoglobin to methaemoglobin. The rates of glycolysis and methaemoglobin reduction were measured following incubation of these cells in buffers containing 1, 5 or 10 mM inorganic phosphate. Glucose consumption and methaemoglobin reduction were increased by inorganic phosphate and were more rapid in those animals supplemented with nitrate. Lactate production of erythrocytes was reduced in those animals fed nitrate. It is concluded that adaptation to chronic nitrite exposure occurs in the erythron, resulting in greater methaemoglobin reduction potential and that there is competition between NADH-methaemoglobin reductase and lactate dehydrogenase for NADH.
Publication Type: Journal Article
Source of Publication: Animal Production Science, 55(5), p. 611-616
Publisher: CSIRO Publishing
Place of Publication: Clayton, Australia
ISSN: 1836-0939
Field of Research (FOR): 070706 Veterinary Medicine
070204 Animal Nutrition
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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