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https://hdl.handle.net/1959.11/21724
Title: | Can adaptation to nitrate supplementation and provision of fermentable energy reduce nitrite accumulation in rumen contents in vitro? | Contributor(s): | de Raphelis-Soissan, Victoire (author); Nolan, John V (author) ; Newbold, J R (author); Godwin, Ian (author); Hegarty, Roger (author) | Publication Date: | 2016 | DOI: | 10.1071/an15609 | Handle Link: | https://hdl.handle.net/1959.11/21724 | Abstract: | Nitrate (NO₃⁻) supplementation is a promising methane mitigation strategy for ruminants, but can cause nitrite (NO₂⁻) poisoning. Because some nitrite reductases are NADH-dependent, we hypothesised that replacing glucose with glycerol would increase the NADH yield and so enhance nitrite reductase activity and reduce ruminal NO₂⁻ accumulation and toxicity risk. We also hypothesised that adapting sheep to dietary NO₃⁻ would limit the accumulation of NO₂⁻ when NO₃⁻ was added to rumen fluid. Changes in NO₃⁻ and NO₂⁻ catabolism and CH₄ production, resulting from supplementation with glycerol to enhance NADH supply, were studied in vitro. In Experiment 1, rumen fluid from sheep adapted to dietary NO₃⁻ (2% of DM intake) or urea (1.1% of DM intake) was incubated with NO₃⁻ or urea, respectively. Additionally, ground oaten hay was added to incubations alone (control), or with glucose or glycerol. In Experiement 2, sheep were adapted for 9 weeks to dietary NO₃⁻ or urea. Nitrate (2% NO₃⁻ of substrate DM) was added to incubated digesta from NO₃⁻ - or urea-supplemented sheep, while urea (1.1% of substrate DM) was added to digesta from urea-supplemented sheep. In both studies, triplicate incubations were terminated at nine time points up to 24 h. Methane emissions were lower in all NO₃⁻ treatments (P < 0.05). Contrary to our hypotheses, both glycerol supplementation (Experiment 1) and prior adaptation to NO₃⁻ (Experiment 2) increased NO₂⁻ accumulation. In Experiment 1, there was no difference in ruminal NO₂⁻ concentration between the unsupplemented control and added glucose treatments. Nitrous oxide accumulated in NO₃⁻ treatments only with rumen fluid from sheep adapted to dietary urea (P < 0.05). In summary, NO₂⁻ accumulation in vitro was not reduced by adaptation to NO₃⁻ or by glucose or glycerol supplementation, disproving the hypotheses regarding the role of NADH availability and of NO₂⁻ adaptation in reducing ruminal NO₂⁻ accumulation and toxicity risk. | Publication Type: | Journal Article | Source of Publication: | Animal Production Science, 56(3), p. 605-612 | Publisher: | CSIRO Publishing | Place of Publication: | Australia | ISSN: | 1836-5787 1836-0939 |
Fields of Research (FoR) 2008: | 070204 Animal Nutrition 070203 Animal Management |
Fields of Research (FoR) 2020: | 300303 Animal nutrition 300302 Animal management |
Socio-Economic Objective (SEO) 2008: | 970105 Expanding Knowledge in the Environmental Sciences 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences |
Socio-Economic Objective (SEO) 2020: | 280111 Expanding knowledge in the environmental sciences 280101 Expanding knowledge in the agricultural, food and veterinary sciences |
Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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