Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21689
Title: Application of qRT-PCR for improved understanding and control of infectious bursal disease in chickens
Contributor(s): Jayasundara, Kanchana Mudiyanselage Kanchana Ganga Kumari (author); Walkden-Brown, Steve  (supervisor)orcid ; Katz, Margaret  (supervisor); Islam, A Fakhrul (supervisor); Renz, Katrin  (supervisor)
Conferred Date: 2017
Copyright Date: 2016
Open Access: Yes
Handle Link: https://hdl.handle.net/1959.11/21689
Abstract: The advent of real time polymerase chain reaction test (qPCR) allows greater diagnostic and research capacity especially beneficial in the field of virology. Infectious bursal disease (IBD) is a well-known viral disease of poultry and occurs world-wide despite widespread use of vaccination. In this thesis study, the real time reverse transcription method (qRT-PCR) was used to address to a range of research questions to provide better understanding and control of IBD in chickens in Australia and world-wide. One experiment was conducted to define the differences between Australian endemic classical and variant infectious bursal disease virus (IBDV) strains by infecting maternal antibody (MAb) free specific pathogen free (SPF) chickens separately with classical strain 06/95 and variant strain 02/95 in isolators. The results revealed that the two IBDV strains did not differ in the degree of bursal atrophy induced, IBDV viral RNA (vRNA) load in bursal and non-bursal lymphoid organs and faecal shedding but variant strain 02/95 induced a greater antibody response to the infection than classical strain 06/95 which was associated with a more rapid decline in IBDV vRNA genome copy number (VCN) in lymphoid organs.
Publication Type: Thesis Doctoral
Field of Research Codes: 070712 Veterinary Virology
070703 Veterinary Diagnosis and Diagnostics
070205 Animal Protection (Pests and Pathogens)
Rights Statement: Copyright 2016 - Kanchana Mudiyanselage Kanchana Ganga Kumari Jayasundara
HERDC Category Description: T2 Thesis - Doctorate by Research
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Appears in Collections:School of Environmental and Rural Science
Thesis Doctoral

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