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Title: Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs
Contributor(s): Crawford, Kimberly (author); Lager, Kelly (author); Miller, Laura (author); Opriessnig, Tanja (author); Gerber, Priscilla Freitas  (author)orcid ; Hesse, Richard (author)
Publication Date: 2015
Open Access: Yes
DOI: 10.1186/s13567-015-0180-5Open Access Link
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Abstract: Clinical disease associated with porcine epidemic diarrhea virus (PEDV) infection in naïve pigs is well chronicled; however, information on endemic PEDV infection is limited. To characterize chronic PEDV infection, the duration of infectious virus shedding and development of protective immunity was determined. On Day 0 (D0), a growing pig was challenged with PEDV and 13 contacts were commingled. On D7, 9 contact pigs (principal virus group (PG)), were selected, moved to a separate room and commingled with one sentinel pig (S1). This process was repeated weekly with S2, S3 and S4. The PG was PEDV-positive by PCR from D3-11, with some pigs intermittently positive to D42. Pigs S1 and S2 were PEDV-positive within 24 hours of commingling. Antibodies were detected in all PG by D21 and by 7 days post-contact in S1 and S2. Pigs S3 and S4 were PCR and antibody negative following commingling. To evaluate protective immunity, 5 naïve pigs (N) and the PG were challenged (N/C, PG/C) with homologous virus on D49. All N/C pigs were PEDV PCR-positive by D52 with detection out to D62 in 3/5 N/C pigs. All PG/C pigs were PEDV PCR-negative post-challenge. By D63, all N/C seroconverted. Although PEDV RNA was demonstrated in pigs after primary infection until D42, infectious PEDV capable of horizontal transmission to naïve pigs was only shed 14-16 days after infection to age-matched pigs. Homologous re-challenge 49 days post initial PEDV exposure did not result in re-infection of the pigs. This demonstrates potential for an effective PEDV vaccine.
Publication Type: Journal Article
Source of Publication: Veterinary Research, v.46, p. 1-9
Publisher: BioMed Central Ltd
Place of Publication: United Kingdom
ISSN: 1297-9716
Field of Research (FOR): 070712 Veterinary Virology
070705 Veterinary Immunology
Socio-Economic Objective (SEO): 830308 Pigs
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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