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https://hdl.handle.net/1959.11/21333
Title: | Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs | Contributor(s): | Crawford, Kimberly (author); Lager, Kelly (author); Miller, Laura (author); Opriessnig, Tanja (author); Gerber, Priscilla Freitas (author) ; Hesse, Richard (author) | Publication Date: | 2015 | Open Access: | Yes | DOI: | 10.1186/s13567-015-0180-5 | Handle Link: | https://hdl.handle.net/1959.11/21333 | Abstract: | Clinical disease associated with porcine epidemic diarrhea virus (PEDV) infection in naïve pigs is well chronicled; however, information on endemic PEDV infection is limited. To characterize chronic PEDV infection, the duration of infectious virus shedding and development of protective immunity was determined. On Day 0 (D0), a growing pig was challenged with PEDV and 13 contacts were commingled. On D7, 9 contact pigs (principal virus group (PG)), were selected, moved to a separate room and commingled with one sentinel pig (S1). This process was repeated weekly with S2, S3 and S4. The PG was PEDV-positive by PCR from D3-11, with some pigs intermittently positive to D42. Pigs S1 and S2 were PEDV-positive within 24 hours of commingling. Antibodies were detected in all PG by D21 and by 7 days post-contact in S1 and S2. Pigs S3 and S4 were PCR and antibody negative following commingling. To evaluate protective immunity, 5 naïve pigs (N) and the PG were challenged (N/C, PG/C) with homologous virus on D49. All N/C pigs were PEDV PCR-positive by D52 with detection out to D62 in 3/5 N/C pigs. All PG/C pigs were PEDV PCR-negative post-challenge. By D63, all N/C seroconverted. Although PEDV RNA was demonstrated in pigs after primary infection until D42, infectious PEDV capable of horizontal transmission to naïve pigs was only shed 14-16 days after infection to age-matched pigs. Homologous re-challenge 49 days post initial PEDV exposure did not result in re-infection of the pigs. This demonstrates potential for an effective PEDV vaccine. | Publication Type: | Journal Article | Source of Publication: | Veterinary Research, v.46, p. 1-9 | Publisher: | BioMed Central Ltd | Place of Publication: | United Kingdom | ISSN: | 1297-9716 0928-4249 |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070705 Veterinary Immunology |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300906 Veterinary immunology |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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