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https://hdl.handle.net/1959.11/21281
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DC Field | Value | Language |
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dc.contributor.author | Gerber, Priscilla Freitas | en |
dc.contributor.author | Pinto, Flavia F | en |
dc.contributor.author | Heinemann, Marcos B | en |
dc.contributor.author | Lobato, Zelia I P | en |
dc.date.accessioned | 2017-06-06T14:24:00Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Pesquisa Veterinária Brasileira, 30(11), p. 918-920 | en |
dc.identifier.issn | 1678-5150 | en |
dc.identifier.issn | 0100-736X | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/21281 | - |
dc.description.abstract | The dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars. | en |
dc.language | en | en |
dc.publisher | Colegio Brasileiro de Patologia Animal | en |
dc.relation.ispartof | Pesquisa Veterinária Brasileira | en |
dc.title | Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1590/S0100-736X2010001100004 | en |
dcterms.accessRights | Gold | en |
dc.subject.keywords | Veterinary Medicine | en |
dc.subject.keywords | Veterinary Diagnosis and Diagnostics | en |
dc.subject.keywords | Veterinary Virology | en |
local.contributor.firstname | Priscilla Freitas | en |
local.contributor.firstname | Flavia F | en |
local.contributor.firstname | Marcos B | en |
local.contributor.firstname | Zelia I P | en |
local.subject.for2008 | 070712 Veterinary Virology | en |
local.subject.for2008 | 070703 Veterinary Diagnosis and Diagnostics | en |
local.subject.for2008 | 070706 Veterinary Medicine | en |
local.subject.seo2008 | 830308 Pigs | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | pgerber2@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-chute-20170602-153810 | en |
local.publisher.place | Brazil | en |
local.format.startpage | 918 | en |
local.format.endpage | 920 | en |
local.identifier.scopusid | 78651347420 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 30 | en |
local.identifier.issue | 11 | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Gerber | en |
local.contributor.lastname | Pinto | en |
local.contributor.lastname | Heinemann | en |
local.contributor.lastname | Lobato | en |
dc.identifier.staff | une-id:pgerber2 | en |
local.booktitle.translated | Brazilian Journal of Veterinary Research | en |
local.profile.orcid | 0000-0002-8343-8299 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:21474 | en |
local.identifier.handle | https://hdl.handle.net/1959.11/21281 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Gerber, Priscilla Freitas | en |
local.search.author | Pinto, Flavia F | en |
local.search.author | Heinemann, Marcos B | en |
local.search.author | Lobato, Zelia I P | en |
local.uneassociation | Unknown | en |
local.year.published | 2010 | en |
Appears in Collections: | Journal Article |
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