Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21280
Title: Comparison of commercial enzyme-linked immunosorbent assays and fluorescent microbead immunoassays for detection of antibodies against porcine reproductive and respiratory syndrome virus in boars
Contributor(s): Gerber, Priscilla Freitas  (author)orcid ; Gimenez-Lirola, Luis G (author); Halbur, Patrick G (author); Zhou, Lei (author); Meng, Xiang-Jin (author); Opriessnig, Tanja (author)
Publication Date: 2014
DOI: 10.1016/j.jviromet.2013.12.001
Handle Link: https://hdl.handle.net/1959.11/21280
Abstract: The objective of this study was to compare the ability of two commercial enzyme-linked immunosorbent assays (ELISAs) and an 'in-house' fluorescent microbead immunoassay (FMIA) to detect IgG antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) types 1 and 2 in serum and oral fluids from boars infected experimentally. Samples from uninfected control pigs and PRRSV-negative field samples were also used. Serum samples were tested by ELISAs (IDEXX Se, HIPRA Se) and an 'in-house' FMIA-Se for detection of PRRSV types 1 and 2. Oral fluids were tested by ELISAs (IDEXX-SO, IDEXX-OF, HIPRA-OF) for detection of PRRSV types 1 and 2. Among the sera, IDEXX-Se and HIPRA-Se had similar sensitivity and specificity (p > 0.05); however, IDEXX-Se detected positive animals earlier than HIPRA-Se (p < 0.05). FMIA-Se had the highest false-positive rates in known negative field samples (1/205 for IDEXX-Se, 5/205 for HIPRA-Se, and 37/205 for FMIA-Se; p < 0.01). Serum and oral fluid samples had similar detection rates and antibody kinetics using the IDEXX tests. There was a higher detection rate in serum than oral fluid using the HIPRA assays. In this study, the nucleocapsid protein utilized as antigen in the FMIAs yielded a low specificity. IDEXX-Se had the earliest detection and similar sensitivity and specificity to the HIPRA-Se.
Publication Type: Journal Article
Source of Publication: Journal of Virological Methods, v.197, p. 63-66
Publisher: Elsevier BV
Place of Publication: Netherlands
ISSN: 1879-0984
0166-0934
Fields of Research (FoR) 2008: 070703 Veterinary Diagnosis and Diagnostics
070705 Veterinary Immunology
070712 Veterinary Virology
Fields of Research (FoR) 2020: 300904 Veterinary diagnosis and diagnostics
300906 Veterinary immunology
300914 Veterinary virology
Socio-Economic Objective (SEO) 2008: 830308 Pigs
Socio-Economic Objective (SEO) 2020: 100410 Pigs
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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