Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21269
Title: Detection of antibodies against porcine epidemic diarrhea virus in serum and colostrum by indirect ELISA
Contributor(s): Gerber, Priscilla Freitas  (author)orcid ; Gong, Qiaoling (author); Huang, Yao-Wei (author); Wang, Chong (author); Holtkamp, Derald (author); Opriessnig, Tanja (author)
Publication Date: 2014
DOI: 10.1016/j.tvjl.2014.07.018
Handle Link: https://hdl.handle.net/1959.11/21269
Abstract: An indirect porcine epidemic diarrhea virus (PEDV) anti-immunoglobulin (Ig) G ELISA based on the S1 portion of the spike protein was validated and compared with an indirect immunofluorescence assay. On field serum samples the diagnostic sensitivity of the S1 ELISA was 100%, the diagnostic specificity was 94% and the S1 ELISA showed no cross-reactivity with antibodies against other porcine coronaviruses. Colostrum samples (n = 133) were also tested for anti-PEDV IgG and IgA. The diagnostic sensitivity was 92% for IgG and 100% for IgA, and the diagnostic specificity was 90% for IgG and 99.4% for IgA. These data suggest that the S1 ELISA is a sensitive and specific test that could also be used to evaluate PEDV colostral immunity.
Publication Type: Journal Article
Source of Publication: The Veterinary Journal, 202(1), p. 33-36
Publisher: Elsevier Ltd
Place of Publication: United Kingdom
ISSN: 1532-2971
1090-0233
Fields of Research (FoR) 2008: 070706 Veterinary Medicine
070712 Veterinary Virology
070703 Veterinary Diagnosis and Diagnostics
Fields of Research (FoR) 2020: 300907 Veterinary medicine (excl. urology)
300914 Veterinary virology
300904 Veterinary diagnosis and diagnostics
Socio-Economic Objective (SEO) 2008: 830308 Pigs
Socio-Economic Objective (SEO) 2020: 100410 Pigs
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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