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https://hdl.handle.net/1959.11/21262
Title: | A PCV2 vaccine based on genotype 2b is more effective than a 2a-based vaccine to protect against PCV2b or combined PCV2a/2b viremia in pigs with concurrent PCV2, PRRSV and PPV infection | Contributor(s): | Opriessnig, Tanja (author); O'Neill, Kevin (author); Gerber, Priscilla Freitas (author) ; de Castro, Alessandra M M G (author); Gimenez-Lirola, Luis G (author); Beach, Nathan M (author); Zhou, Lei (author); Meng, Xiang-Jin (author); Wang, Chong (author); Halbur, Patrick G (author) | Publication Date: | 2013 | DOI: | 10.1016/j.vaccine.2012.11.030 | Handle Link: | https://hdl.handle.net/1959.11/21262 | Abstract: | The predominant genotype of porcine circovirus (PCV) in the pig population today is PCV2b yet PCV2a-based commercial vaccines are considered effective in protecting against porcine circovirus associated disease. The objective of this study was to compare the ability of PCV2a- and PCV2b-based vaccines to control PCV2b viremia in a challenge model that mimics the U.S. field situation. Sixty-three pigs were randomly assigned to one of eight groups. Sixteen pigs were vaccinated with an experimental live-attenuated chimeric PCV1-2a vaccine based on genotype 2a and another 16 pigs with a chimeric PCV1-2b vaccine based on genotype 2b. Challenge was done 28 days post vaccination (dpv) using PCV2b (or a combination of PCV2a and PCV2b), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV) to mimic what commonly occurs in the field. The experiment was terminated 21 days post challenge (dpc) or 49 dpv. Pigs vaccinated with the chimeric PCV1-2b vaccine had significantly higher levels of PCV1-2b viremia and shedding of the PCV1-2b vaccine virus in feces and nasal secretions but also a more robust humoral immune response as evidenced by significantly higher ELISA S/P ratios compared to the PCV1-2a vaccination. Regardless of challenge, the PCV1-2b vaccination significantly reduced the prevalence and amount of PCV2 viremia compared to the PCV1-2a vaccination. Interestingly, in the non-vaccinated pigs concurrent PCV2a infection resulted in clinical disease and increased macroscopic lung lesions compared to pigs challenged with PCV2b alone, further supporting the idea that concurrent PCV2a/PCV2b infection is necessary for optimal PCV2 replication. | Publication Type: | Journal Article | Source of Publication: | Vaccine, 31(3), p. 487-494 | Publisher: | Elsevier Ltd | Place of Publication: | United Kingdom | ISSN: | 1873-2518 0264-410X |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070706 Veterinary Medicine |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300907 Veterinary medicine (excl. urology) |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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