Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21256
Title: Development of a fluorescent microbead-based immunoassay for the detection of hepatitis E virus IgG antibodies in pigs and comparison to an enzyme-linked immunoassay
Contributor(s): Owolodun, Olajide A (author); Gimenez-Lirola, Luis G (author); Gerber, Priscilla Freitas  (author)orcid ; Sanford, Brenton J (author); Feagins, Alicia R (author); Meng, Xiang-Jin (author); Halbur, Patrick G (author); Opriessnig, Tanja (author)
Publication Date: 2013
DOI: 10.1016/j.jviromet.2013.06.010
Handle Link: https://hdl.handle.net/1959.11/21256
Abstract: Swine hepatitis E virus (HEV) is a zoonotic virus and pigs are considered as an important reservoir. Swine HEV infection is widespread and most pig herds are infected. Humans can be infected with swine HEV via consumption of undercooked pork or through direct contact with infected pigs. To minimize the risk of zoonotic transmission, sensitive tools to assess the HEV infection status of pigs and pork products are needed. The objective of this study was to develop a fluorescent microbead-based immunoassay (FMIA) for the detection of IgG antibodies against swine HEV and compare it to an in-house enzyme-linked immunoassay (ELISA). Three sets of samples were utilized: (A) samples from pigs infected experimentally with different strains of HEV (positive controls, n= 72), (B) samples from known HEV-negative pigs (negative controls, n= 62) and (C) samples from pigs of unknown HEV infection status (n= 182). All samples were tested by both ELISA and FMIA. The results on the experimental samples with known HEV exposure indicate that both assays have a specificity of 100% while the sensitivity ranges from 84.6% (ELISA) to 92.3% (FMIA). The overall prevalence of HEV IgG antibodies in field samples from pigs with unknown HEV exposure was 21.9% (40/182) for the ELISA and 21.4% (39/182) for the FMIA. The two assays had an almost perfect overall agreement (Kappa= 0.92).
Publication Type: Journal Article
Source of Publication: Journal of Virological Methods, 193(2), p. 278-283
Publisher: Elsevier BV
Place of Publication: The Netherlands
ISSN: 0166-0934
1879-0984
Field of Research (FOR): 070712 Veterinary Virology
070706 Veterinary Medicine
070703 Veterinary Diagnosis and Diagnostics
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 26
Views: 759
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
2 files
File Description SizeFormat 
Show full item record

SCOPUSTM   
Citations

2
checked on Nov 26, 2018

Page view(s)

50
checked on May 2, 2019
Google Media

Google ScholarTM

Check

Altmetric


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.