Validation of an immunoperoxidase monolayer assay for total anti-'Vaccinia virus' antibody titration

Title
Validation of an immunoperoxidase monolayer assay for total anti-'Vaccinia virus' antibody titration
Publication Date
2012
Author(s)
Gerber, Priscilla Freitas
( author )
OrcID: https://orcid.org/0000-0002-8343-8299
Email: pgerber2@une.edu.au
UNE Id une-id:pgerber2
Matos, Ana Carolina D
Guedes, Maria Isabel M C
Madureira, Marieta C
Silva, Marcos X
Lobato, Zelia I P
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Sage Publications, Inc
Place of publication
United States of America
DOI
10.1177/1040638711435231
UNE publication id
une:21436
Abstract
'Vaccinia virus' (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were -1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine.
Link
Citation
Journal of Veterinary Diagnostic Investigation, 24(2), p. 355-358
ISSN
1943-4936
1040-6387
Start page
355
End page
358

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