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https://hdl.handle.net/1959.11/21244
Title: | Validation of an immunoperoxidase monolayer assay for total anti-'Vaccinia virus' antibody titration | Contributor(s): | Gerber, Priscilla Freitas (author) ; Matos, Ana Carolina D (author); Guedes, Maria Isabel M C (author); Madureira, Marieta C (author); Silva, Marcos X (author); Lobato, Zelia I P (author) | Publication Date: | 2012 | Open Access: | Yes | DOI: | 10.1177/1040638711435231 | Handle Link: | https://hdl.handle.net/1959.11/21244 | Abstract: | 'Vaccinia virus' (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were -1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine. | Publication Type: | Journal Article | Source of Publication: | Journal of Veterinary Diagnostic Investigation, 24(2), p. 355-358 | Publisher: | Sage Publications, Inc | Place of Publication: | United States of America | ISSN: | 1943-4936 1040-6387 |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070703 Veterinary Diagnosis and Diagnostics 070706 Veterinary Medicine |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300904 Veterinary diagnosis and diagnostics 300907 Veterinary medicine (excl. urology) |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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