Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21244
Title: Validation of an immunoperoxidase monolayer assay for total anti-'Vaccinia virus' antibody titration
Contributor(s): Gerber, Priscilla Freitas  (author)orcid ; Matos, Ana Carolina D (author); Guedes, Maria Isabel M C (author); Madureira, Marieta C (author); Silva, Marcos X (author); Lobato, Zelia I P (author)
Publication Date: 2012
Open Access: Yes
DOI: 10.1177/1040638711435231Open Access Link
Handle Link: https://hdl.handle.net/1959.11/21244
Abstract: 'Vaccinia virus' (VACV) has been associated with zoonotic exanthemic outbreaks affecting bovids and human beings, with significant public health and economic impacts. Rapid and reliable diagnostic methods are needed to detect and epidemiologically monitor antibodies to VACV. The current study describes the development of an immunoperoxidase monolayer assay (IPMA) for detection of total VACV antibodies in bovine serum. The assay was validated by comparison with a plaque reduction neutralization test (PRNT). Kappa index of agreement, diagnostic sensitivity, specificity, and accuracy of the IPMA were -1.008, 100%, 96%, and 98%, respectively, when compared with PRNT on 148 field bovine sera. Repeatability tests on 32 field-positive serum samples revealed that intraclass coefficient correlation was 0.86. In experimentally infected cattle, VACV antibodies were detectable by IPMA 4 days postinfection, which was more than 2 weeks earlier than with the PRNT, indicating that IPMA could be a more sensitive test than the latter. In 4 naturally VACV-diseased cows monitored for 13 months, IPMA could detect VACV antibodies up to 13 months, a longer time than PRNT. The IPMA is simpler to produce and perform when compared with PRNT and is time saving and suitable for large-scale surveys of VACV infection in bovine.
Publication Type: Journal Article
Source of Publication: Journal of Veterinary Diagnostic Investigation, 24(2), p. 355-358
Publisher: Sage Publications Inc
Place of Publication: United States of America
ISSN: 1943-4936
1040-6387
Field of Research (FOR): 070712 Veterinary Virology
070703 Veterinary Diagnosis and Diagnostics
070706 Veterinary Medicine
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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