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Title: Total Synthesis of Homogeneous Variants of Hirudin P6: A Post-Translationally Modified Anti-Thrombotic Leech-Derived Protein
Contributor(s): Hsieh, Yves S Y (author); Wijeyewickrema, Lakshmi C (author); Wilkinson, Brendan  (author)orcid ; Pike, Robert N (author); Payne, Richard J (author)
Publication Date: 2014
DOI: 10.1002/anie.201310777
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Abstract: Hirudin P6 is a leech-derived anti-thrombotic protein which possesses two post-translational modifications, O-glycosylation and tyrosine sulfation. In this study we report the ligation-based synthesis of a library of hirudin P6 proteins possessing homogeneous glycosylation and sulfation modifications. The nature of the modifications incorporated was shown to have a drastic effect on inhibition against both the fibrinogenolytic and amidolytic activities of thrombin and thus highlights a potential means for attenuating the biological activity of the protein.
Publication Type: Journal Article
Source of Publication: Angewandte Chemie (International Edition), 53(15), p. 3947-3951
Publisher: Wiley-VCH Verlag GmbH & Co KGaA
Place of Publication: Germany
ISSN: 1521-3773
Field of Research (FoR) 2008: 030401 Biologically Active Molecules
030406 Proteins and Peptides
030503 Organic Chemical Synthesis
Field of Research (FoR) 2020: 340401 Biologically active molecules
340407 Proteins and peptides
340503 Organic chemical synthesis
Socio-Economic Objective (SEO) 2008: 970103 Expanding Knowledge in the Chemical Sciences
929999 Health not elsewhere classified
970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objective (SEO) 2020: 280105 Expanding knowledge in the chemical sciences
280102 Expanding knowledge in the biological sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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Appears in Collections:Journal Article
School of Science and Technology

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