Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/20676
Title: Total Synthesis of Homogeneous Variants of Hirudin P6: A Post-Translationally Modified Anti-Thrombotic Leech-Derived Protein
Contributor(s): Hsieh, Yves S Y (author); Wijeyewickrema, Lakshmi C (author); Wilkinson, Brendan  (author)orcid ; Pike, Robert N (author); Payne, Richard J (author)
Publication Date: 2014
DOI: 10.1002/anie.201310777
Handle Link: https://hdl.handle.net/1959.11/20676
Abstract: Hirudin P6 is a leech-derived anti-thrombotic protein which possesses two post-translational modifications, O-glycosylation and tyrosine sulfation. In this study we report the ligation-based synthesis of a library of hirudin P6 proteins possessing homogeneous glycosylation and sulfation modifications. The nature of the modifications incorporated was shown to have a drastic effect on inhibition against both the fibrinogenolytic and amidolytic activities of thrombin and thus highlights a potential means for attenuating the biological activity of the protein.
Publication Type: Journal Article
Source of Publication: Angewandte Chemie (International Edition), 53(15), p. 3947-3951
Publisher: Wiley-VCH Verlag GmbH & Co KGaA
Place of Publication: Germany
ISSN: 1521-3773
1433-7851
Fields of Research (FoR) 2008: 030401 Biologically Active Molecules
030406 Proteins and Peptides
030503 Organic Chemical Synthesis
Fields of Research (FoR) 2020: 340407 Proteins and peptides
340401 Biologically active molecules
340503 Organic chemical synthesis
Socio-Economic Objective (SEO) 2008: 970103 Expanding Knowledge in the Chemical Sciences
929999 Health not elsewhere classified
970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objective (SEO) 2020: 280105 Expanding knowledge in the chemical sciences
280102 Expanding knowledge in the biological sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Science and Technology

Files in This Item:
2 files
File Description SizeFormat 
Show full item record

SCOPUSTM   
Citations

34
checked on Mar 9, 2024

Page view(s)

1,058
checked on Mar 7, 2023
Google Media

Google ScholarTM

Check

Altmetric


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.