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Title: Proteomic and Microscopic Strategies towards the Analysis of the Cytoskeletal Networks in Major Neuropsychiatric Disorders
Contributor(s): Coumans-Moens, Joelle  (author)orcid ; Palanisamy, Suresh K A (author); McFarlane, James R (author)orcid ; Moens, Pierre  (author)orcid 
Publication Date: 2016
Open Access: Yes
DOI: 10.3390/ijms17040581Open Access Link
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Abstract: Mental health disorders have become worldwide health priorities. It is estimated that in the next 20 years they will account for a 16 trillion United State dollars (US$) loss. Up to now, the underlying pathophysiology of psychiatric disorders remains elusive. Altered cytoskeleton proteins expression that may influence the assembly, organization and maintenance of cytoskeletal integrity has been reported in major depressive disorders, schizophrenia and to some extent bipolar disorders. The use of quantitative proteomics, dynamic microscopy and super-resolution microscopy to investigate disease-specific protein signatures holds great promise to improve our understanding of these disorders. In this review, we present the currently available quantitative proteomic approaches use in neurology, gel-based, stable isotope-labelling and label-free methodologies and evaluate their strengths and limitations. We also reported on enrichment/subfractionation methods that target the cytoskeleton associated proteins and discuss the need of alternative methods for further characterization of the neurocytoskeletal proteome. Finally, we present live cell imaging approaches and emerging dynamic microscopy technology that will provide the tools necessary to investigate protein interactions and their dynamics in the whole cells. While these areas of research are still in their infancy, they offer huge potential towards the understanding of the neuronal network stability and its modification across neuropsychiatric disorders.
Publication Type: Journal Article
Source of Publication: International Journal of Molecular Sciences, 17(4), p. 1-19
Publisher: MDPIAG
Place of Publication: Switzerland
ISSN: 1422-0067
Field of Research (FOR): 110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
110903 Central Nervous System
110319 Psychiatry (incl. Psychotherapy)
Socio-Economic Outcome Codes: 920410 Mental Health
920112 Neurodegenerative Disorders Related to Ageing
920111 Nervous System and Disorders
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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