Fluxomics - connecting 'omics analysis and phenotypes

Author(s)
Winter, Gal
Kromer, Jens O
Publication Date
2013
Abstract
In our modern 'omics era, metabolic flux analysis (fluxomics) represents the physiological counterpart of its siblings transcriptomics, proteomics and metabolomics. Fluxomics integrates 'in vivo' measurements of metabolic fluxes with stoichiometric network models to allow the determination of absolute flux through large networks of the central carbon metabolism. There are many approaches to implement fluxomics including flux balance analysis (FBA), ¹³C fluxomics and ¹³C-constrained FBA as well as many experimental settings for flux measurement including dynamic, stationary and semi-stationary. Here we outline the principles of the different approaches and their relative advantages. We demonstrate the unique contribution of flux analysis for phenotype elucidation using a thoroughly studied metabolic reaction as a case study, the microbial aerobic/anaerobic shift, highlighting the importance of flux analysis as a single layer of data as well as interlaced in multi-omics studies.
Citation
Environmental Microbiology, 15(7), p. 1901-1916
ISSN
1462-2920
1462-2912
Link
Publisher
Wiley-Blackwell Publishing Ltd
Title
Fluxomics - connecting 'omics analysis and phenotypes
Type of document
Journal Article
Entity Type
Publication

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