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https://hdl.handle.net/1959.11/20482
Title: | 'In situ' high throughput method for H2S detection during micro-scale wine fermentation | Contributor(s): | Winter, Gal (author) ; Curtin, Chris (author) | Publication Date: | 2012 | DOI: | 10.1016/j.mimet.2012.08.003 | Handle Link: | https://hdl.handle.net/1959.11/20482 | Abstract: | An 'in situ' high throughput method for the detection of H₂S during fermentation was developed. The method utilizes a redox reaction in which sulfide ion reduces methylene blue, leading to its decolourisation. Incorporation of methylene blue into the fermentation media allows real-time detection of H₂S during fermentation and the generation of an H₂S production profile. Kinetic parameters extracted from the H₂S production profile can be used to characterise genetic factors affecting H₂S production and differentiate between environmental conditions affecting it. The method, validated here for 'Saccharomyces cerevisiae', is suited for high throughput screening purposes by virtue of its simplicity and the ability to detect H₂S in micro-scale fermentations. | Publication Type: | Journal Article | Source of Publication: | Journal of Microbiological Methods, 91(1), p. 165-170 | Publisher: | Elsevier BV | Place of Publication: | Netherlands | ISSN: | 1872-8359 0167-7012 |
Fields of Research (FoR) 2008: | 060104 Cell Metabolism | Fields of Research (FoR) 2020: | 310103 Cell metabolism | Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences | Socio-Economic Objective (SEO) 2020: | 280102 Expanding knowledge in the biological sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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