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https://hdl.handle.net/1959.11/20176
Title: | Muscle-Enriched MicroRNAs Isolated from Whole Blood Are Regulated by Exercise and Are Potential Biomarkers of Cardiorespiratory Fitness | Contributor(s): | Denham, Joshua (author); Prestes, Priscilla R (author) | Publication Date: | 2016 | Open Access: | Yes | DOI: | 10.3389/fgene.2016.00196 | Handle Link: | https://hdl.handle.net/1959.11/20176 | Abstract: | MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally. Evidence indicating miRNAs influence exercise-induced health and performance adaptations is mounting. Circulating miRNAs are responsible for intercellular communication and could serve as biomarkers for disease and exercise-related traits. Such biomarkers would contribute to exercise screening, monitoring, and the development of personalized exercise prescription. Accordingly, we investigated the impact of long-term strenuous aerobic exercise training and a single bout of maximal aerobic exercise on five muscle-enriched miRNAs implicated in exercise adaptations (miR-1, miR-133a, miR-181a, miR-486, and miR-494). We also determined linear correlations between miRNAs, resting heart rate, and maximum oxygen uptake (VO₂max). We used TaqMan assay quantitative polymerase chain reaction to analyze the abundance of miR-1, miR-133a, miR-181a, miR-486, and miR-494 in resting whole blood of 67 endurance athletes and 61 healthy controls. Relative to controls, endurance athletes exhibited increased miR-1, miR-486, and miR-494 content (1.26- to 1.58-fold change, all p < 0.05). miR-1, miR-133a, and miR-486 were decreased immediately after maximal aerobic exercise (0.64- to 0.76-fold change, all p < 0.01) performed by 19 healthy, young men (20.7 ± 2.4 years). Finally, we observed positive correlations between miRNA abundance and www.frontiersin.orgO2 max (miR-1 and miR-486) and an inverse correlation between miR-486 and resting heart rate. Therefore, muscle-enriched miRNAs isolated from whole blood are regulated by acute and long-term aerobic exercise training and could serve as biomarkers of cardiorespiratory fitness. | Publication Type: | Journal Article | Source of Publication: | Frontiers in Genetics, v.7, p. 1-8 | Publisher: | Frontiers Research Foundation | Place of Publication: | Switzerland | ISSN: | 1664-8021 | Fields of Research (FoR) 2008: | 110602 Exercise Physiology 060404 Epigenetics (incl. Genome Methylation and Epigenomics) 060405 Gene Expression (incl. Microarray and other genome-wide approaches) |
Fields of Research (FoR) 2020: | 420702 Exercise physiology 310504 Epigenetics (incl. genome methylation and epigenomics) 310505 Gene expression (incl. microarray and other genome-wide approaches) |
Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences 970111 Expanding Knowledge in the Medical and Health Sciences |
Socio-Economic Objective (SEO) 2020: | 280102 Expanding knowledge in the biological sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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