Photomodulation of bacterial growth and biofilm formation using carbohydrate-based surfactants

Title
Photomodulation of bacterial growth and biofilm formation using carbohydrate-based surfactants
Publication Date
2016
Author(s)
Hu, Yingxue
Zou, Wenyue
Julita, Villy
Ramanathan, Rajesh
Tabor, Rico F
Nixon-Luke, Reece
Bryant, Gary
Bansal, Vipul
Wilkinson, Brendan
( author )
OrcID: https://orcid.org/0000-0003-1866-6540
Email: bwilkin7@une.edu.au
UNE Id une-id:bwilkin7
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Royal Society of Chemistry
Place of publication
United Kingdom
DOI
10.1039/c6sc03020c
UNE publication id
une:20207
Abstract
Naturally occurring and synthetic carbohydrate amphiphiles have emerged as a promising class of antimicrobial and antiadhesive agents that act through a number of dynamic and often poorly understood mechanisms. In this paper, we provide the first report on the application of azobenzene trans-cis photoisomerization for effecting spatial and temporal control over bacterial growth and biofilm formation using carbohydrate-based surfactants. Photocontrollable surface tension studies and small angle neutron scattering (SANS) revealed the diverse geometries and dimensions of self-assemblies (micelles) made possible through variation of the head group and UV-visible light irradiation. Using these light-addressable amphiphiles, we demonstrate optical control over the antibacterial activity and formation of biofilms against multi-drug resistant (MDR) 'Pseudomonas aeruginosa', methicillin-resistant 'Staphylococcus aureus' (MRSA) and Gram-negative 'Escherichia coli'. To probe the mechanism of bioactivity further, we evaluated the impact of trans-cis photoisomerization in these surfactants on bacterial motility and revealed photomodulated enhancement in swarming motility in 'P. aeruginosa'. These light-responsive amphiphiles should attract significant interest as a new class of antibacterial agents and as investigational tools for probing the complex mechanisms underpinning bacterial adhesion and biofilm formation.
Link
Citation
Chemical Science, 7(11), p. 6628-6634
ISSN
2041-6539
2041-6520
Start page
6628
End page
6634

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