Contribution of intestinal- and cereal-derived phytase activity on phytate degradation in young broilers

Title
Contribution of intestinal- and cereal-derived phytase activity on phytate degradation in young broilers
Publication Date
2015
Author(s)
Morgan, Natalie
( author )
OrcID: https://orcid.org/0000-0002-9663-2365
Email: nmorga20@une.edu.au
UNE Id une-id:nmorga20
Walk, C L
Bedford, M R
Burton, E J
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Oxford University Press
Place of publication
United States of America
DOI
10.3382/ps/pev108
UNE publication id
une:20175
Abstract
There is little consensus as to the capability of poultry to utilize dietary phytate without supplemental phytase. Therefore, an experiment was conducted to examine the extent to which endogenous phytase of intestinal and cereal origin contributes to phytate degradation in birds aged 0 to 14 d posthatch. Ross 308 broilers (n = 720) were fed one of 4 experimental diets with differing dietary ingredient combinations and approximate total phytate levels of 10 g/kg, dietary phytase activity analyzed at 460 U/kg, dietary calcium (Ca) levels of 11 g/kg, and nonphytate-phosphorus (P) levels of 4 g/kg. Broiler performance, gizzard, duodenum, jejunum and ileum pH, Ca and P digestibility and solubility, amount of dietary phytate hydrolyzed in the gizzard, jejunum, and ileal digesta phytase activity were analyzed at d 4, 6, 8, 10, 12, and 14 posthatch. Intestinal endogenous phytase activity increased significantly (P < 0.001) between d 4 and 6, resulting in increased phytate hydrolysis in the gizzard (P = 0.003), jejunum (P < 0.001), and ileum (P < 0.001). Phytase activity and phytate hydrolysis continued to increase with age, with a greater phytase activity and associated increase in phytate hydrolysis and mineral utilization between d 10 and 12. Gizzard and jejunum Ca and P solubility and ileal Ca and P digestibility increased significantly (P < 0.001), and gastrointestinal pH decreased significantly (P < 0.001) between d 4 and 6. By d 14, phytase activity recovered in the ileum was approximately 45 U/kg. There were strong correlations between phytase activity measured in the ileum and phytate hydrolyzed in the gizzard (r = 0.905, P < 0.001), jejunum (r = 0.901, P = 0.023), and ileum (r = 0.938, P = 0.042). This study shows intestinal- and dietary-derived endogenous phytase activity is responsible for phytate-P hydrolysis in broilers.
Link
Citation
Poultry Science, 94(7), p. 1577-1583
ISSN
1525-3171
0032-5791
Start page
1577
End page
1583

Files:

NameSizeformatDescriptionLink