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Title: RNA Sequencing Applied to Livestock Production
Contributor(s): de las Heras-Saldana, Sara (author); Al-Mamun, Hawlader A (author); Ferdosi, Mohammad (author); Khansefid, Majid (author); Gondro, Cedric (author)orcid 
Publication Date: 2016
DOI: 10.1007/978-3-319-43335-6_4
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Abstract: High-throughput sequencing technology is rapidly replacing expression arrays and becoming the standard method for global expression profiling studies. The development of low-cost, rapid sequencing technologies has enabled detailed quantification of gene expression levels, affecting almost every field in the life sciences. In this chapter, we will overview the key points for gene expression analysis using RNA-seq data. First, we will discuss the workflows of RNA-seq data analysis followed by a discussion about the currently available tools for data analysis and a comparison between these tools. The chapter concludes with a discussion about the application of RNA-seq data analysis in livestock. In the appendix, using an example from livestock RNA-seq data, we show a simple script for RNA-seq data analysis.
Publication Type: Book Chapter
Grant Details: ARC/DP130100542
Source of Publication: Systems Biology in Animal Production and Health, v.1, p. 63-94
Publisher: Springer International Publishing
Place of Publication: Cham, Switzerland
ISBN: 9783319433332
Field of Research (FOR): 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
070201 Animal Breeding
HERDC Category Description: B1 Chapter in a Scholarly Book
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