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Title: A Simplified, Sequential, Phosphorus Fractionation Method
Contributor(s): Guppy, Christopher  (author)orcid ; Menzies, N W (author); Moody, P W (author); Compton, B L (author); Blamey, F P C (author)
Publication Date: 2000
Open Access: Yes
DOI: 10.1080/00103620009370556
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Abstract: Hedley et al. (1982) developed what has become the most widely used (and modified), phosphorus (P) fractionation technique. It consists of sequential extraction of increasingly less phytoavailable P pools. Extracts are centrifuged at up to 25000 g (RCF) and filtered to 0.45 μm to ensure that soil is not lost between extractions. In attempting to transfer this method to laboratories with limited facilities, it was considered that access to high-speed centrifuges, and the cost of frequent filtration may prevent adoption of this P fractionation technique. The modified method presented here was developed to simplify methodology, reduce cost, and therefore increase accessibility of P fractionation technology. It provides quantitative recovery of soil between extractions, using low speed centrifugation without filtration. This is achieved by increasing the ionic strength of dilute extracts, through the addition of NaCl, to flocculate clay particles. Addition of NaCl does not change the amount of P extracted. Flocculation with low speed centrifugation produced extracts comparable with those having undergone filtration (0.025 μm). A malachite green colorimetric method was adopted for inorganic P determination, as this simple manual method provides high sensitivity with negligible interference from other anions. This approach can also be used for total P following digestion, alternatively non-discriminatory methods, such as inductively coupled plasma atomic emission spectroscopy, may be employed.
Publication Type: Journal Article
Source of Publication: Communications in Soil Science and Plant Analysis, 31(11-14), p. 1981-1991
Publisher: Taylor & Francis Inc
Place of Publication: New York, United States of America
ISSN: 1532-2416
Field of Research (FOR): 050304 Soil Chemistry (excl. Carbon Sequestration Science)
Socio-Economic Outcome Codes: 961402 Farmland, Arable Cropland and Permanent Cropland Soils
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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