Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/19684
Title: Molecular analyses provide insight into mechanisms underlying sarcopenia and myofibre denervation in old skeletal muscles of mice
Contributor(s): Barns, Mitchell (author); Gondro, Cedric  (author)orcid ; Tellam, Ross L (author); Radley-Crabb, Hannah G (author); Grounds, Miranda D (author); Shavlakadze, Tea (author)
Publication Date: 2014
DOI: 10.1016/j.biocel.2014.04.025
Handle Link: https://hdl.handle.net/1959.11/19684
Abstract: Molecular mechanisms that are associated with age-related denervation and loss of skeletal muscle mass and function (sarcopenia) are described for female C57Bl/6J mice aged 3, 15, 24, 27 and 29 months (m). Changes in mRNAs and proteins associated with myofibre denervation and protein metabolism in ageing muscles are reported, across the transition from healthy adult myofibres to sarcopenia that occurs between 15 and 24 m. This onset of sarcopenia at 24 m, corresponded with increased expression of genes associated with neuromuscular junction denervation including Chnrg, Chrnd, Ncam1, Runx1, Gadd45a and Myog. Sarcopenia in quadriceps muscles also coincided with increased protein levels for Igf1 receptor, Akt and ribosomal protein S6 (Rps6) with increased phosphorylation of Rps6 (Ser235/236) and elevated Murf1 mRNA and protein, but not Fbxo32: many of these changes are also linked to denervation. Global transcription profiling via microarray analysis confirmed these functional themes and highlighted additional themes that may be a consequence of pathology associated with sarcopenia, including changes in fatty acid metabolism, extracellular matrix structure and protein catabolism. Ageing was also associated with increased global gene expression variance, consistent with decreased control of gene regulation.
Publication Type: Journal Article
Grant Details: ARC/LP120100520
Source of Publication: International Journal of Biochemistry and Cell Biology, v.53, p. 174-185
Publisher: Pergamon Press
Place of Publication: United Kingdom
ISSN: 1878-5875
1357-2725
Field of Research (FOR): 060405 Gene Expression (incl. Microarray and other genome-wide approaches)
Socio-Economic Outcome Codes: 920116 Skeletal System and Disorders (incl. Arthritis)
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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