A Loop Isothermal Amplification Assay to Detect Fowl Adenovirus -8 in a Variety of Poultry Materials

Abstract

A variety of molecular tests to detect avian adenoviruses are available, but they are either time-consuming or not specific to Fowl adenovirus serotype 8 (FAdV-8). Therefore, the aim was to develop a loop isothermal amplification (LAMP) assay to specifically detect FAdV-8 in a variety of poultry samples, particularly dust, faeces and litter. The LAMP assay is a particularly rapid, robust and a low cost molecular test yet with a sensitivity comparable with the real-time PCR technique. Samples were analysed from chickens experimentally infected with FAdV-8 and derived from both tissues and environmental samples, including dust, litter and faeces. The LAMP results matched with those obtained from a generic FAdV real-time PCR test and the LAMP test has shown no cross-reactivity with FAdV serotypes 2, 9, or 11.