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Title: A Loop Isothermal Amplification Assay to Detect Fowl Adenovirus -8 in a Variety of Poultry Materials
Contributor(s): Renz, Katrin  (author); Achari, Robin  (author); Walkden-Brown, Steve W  (author)orcid ; Katz, Margaret E  (author)
Publication Date: 2015
Open Access: Yes
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Abstract: A variety of molecular tests to detect avian adenoviruses are available, but they are either time-consuming or not specific to Fowl adenovirus serotype 8 (FAdV-8). Therefore, the aim was to develop a loop isothermal amplification (LAMP) assay to specifically detect FAdV-8 in a variety of poultry samples, particularly dust, faeces and litter. The LAMP assay is a particularly rapid, robust and a low cost molecular test yet with a sensitivity comparable with the real-time PCR technique. Samples were analysed from chickens experimentally infected with FAdV-8 and derived from both tissues and environmental samples, including dust, litter and faeces. The LAMP results matched with those obtained from a generic FAdV real-time PCR test and the LAMP test has shown no cross-reactivity with FAdV serotypes 2, 9, or 11.
Publication Type: Conference Publication
Conference Name: 26th Annual Australian Poultry Science Symposium, Sydney, Australia, 9th - 11th February, 2015
Conference Details: 26th Annual Australian Poultry Science Symposium, Sydney, Australia, 9th - 11th February, 2015
Source of Publication: Proceedings of the 26th Annual Australian Poultry Science Symposium, p. 171-174
Publisher: Poultry Research Foundation, University of Sydney
Place of Publication: Sydney, Australia
ISSN: 1034-6260
Field of Research (FOR): 070712 Veterinary Virology
070709 Veterinary Pathology
Socio-Economic Outcome Codes: 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
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Series Name: Australian Poultry Science Symposium Proceedings
Series Number : 26
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Appears in Collections:Conference Publication
School of Environmental and Rural Science

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